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Updated: Jun 3, 2026

Experimental Approaches for Biochemical Analysis of Glial Fibrillary Acidic Protein and Its Disease-associated Variants
Published on: November 28, 2025
The identification of novel protein, brain-derived integrating factor-1 (BDIF1), which interacts with astrocytic gap
Takeshi Ito1, Takatoshi Ueki, Hiromu Furukawa
1Hamamatsu University School of Medicine, Department of Anatomy and Neuroscience, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan.
Abstract:
Accumulating evidence demonstrates astrocytic crucial roles in the brain, but the molecular basis underlying astrocytic intracellular protein trafficking remains to be elucidated. The present study reports the identification of novel protein, brain-derived integrating factor-1 (BDIF1), which comprises TBC (Tre-2/Bub2/Cdc16), SH3, RUN domains. The amino acid sequence putatively coding TBC domain in BDIF1 implied its potential to interact with small GTP-binding proteins (G-proteins), and further analyses by co-immunprecipitation and immunocytochemical staining demonstrated that BDIF1 bound to astrocytic gap junctional protein, connexin-43 (Cx43). Our present data shows that BDIF1 potentially functions in molecular trafficking in astrocytes.
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