Antioxidant activity of Convolvulus hystrix Vahl and its chemical constituents
Abd El Raheim Mohammed Donia1, Saleh Ibrahim Alqasoumi, Amani Shafeek Awaad
1Medicinal and Aromatic Plants Department, Desert Research Center, Cairo, Egypt.
Abstract:
Total alcohol and successive extracts (diethyl ether, chloroform, ethyl acetate and n-butanol) of Covolvulus hystrix Vahl were screened for their antioxidant activity using free radical 2-2-diphenyl-1-picrylhydrazyl (DPPH) and Trolox as reference substance. The tested extracts showed variable antioxidant activities. The highest antioxidant activity was achieved in total alcohol (48.32%) followed by ethyl acetate (37.8%) and n-butanol (32.96%) extracts. From the ethyl acetate extract four compounds were isolated and identified as quercetin, quercetin 3-0-rutinoside and quercetin-7-O-rhamnoside in addition to ferulic acid using different spectroscopic analysis such as, 1HNMR, 13CNMR, COSY, HMBC and HMQC.
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