Related Experiment Video

Updated: Jun 3, 2026

Tropomodulin 3 Overexpression as a Marker for Platinum Resistance and Immune Infiltration in Ovarian Cancer
09:40

Tropomodulin 3 Overexpression as a Marker for Platinum Resistance and Immune Infiltration in Ovarian Cancer

Published on: August 2, 2024

Dysfunction of volume-sensitive chloride channels contributes to cisplatin resistance in human lung adenocarcinoma

Xian-Jun Min1, Hui Li, Sheng-Cai Hou

  • 1Beijing Key Laboratory of Respiratory and Pulmonary Circulation Disorders, Capital Medical University, Beijing 100069, China.

Experimental Biology and Medicine (Maywood, N.J.)
|April 2, 2011
PubMed

Insights

Impaired volume-sensitive outward rectifying (VSOR) chloride channels contribute to cisplatin resistance in lung cancer. Restoring VSOR channel activity may overcome drug resistance.

Area of Science:

  • Cell Biology
  • Molecular Pharmacology
  • Oncology

Background:

  • Cisplatin is a standard non-small cell lung cancer treatment.
  • Drug resistance limits cisplatin efficacy, with mechanisms not fully understood.

Purpose of the Study:

  • Investigate the role of volume-sensitive Cl(-) channels in cisplatin resistance.
  • Examine these channels in human lung adenocarcinoma (A549) cells.

Main Methods:

  • Patch-clamp recording
  • Cell volume measurement
  • Apoptosis assays
  • A549 and cisplatin-resistant A549/CDDP cells

Main Results:

  • Cisplatin induced apoptotic volume decrease (AVD) and VSOR Cl(-) current in wild-type A549 cells.
  • Cisplatin-resistant A549/CDDP cells showed impaired AVD and VSOR Cl(-) current.
  • Trichostatin A partially restored VSOR Cl(-) current and enhanced apoptosis in A549/CDDP cells.

Conclusions:

  • Impaired VSOR Cl(-) channel activity contributes to cisplatin resistance in A549/CDDP cells.
  • Targeting VSOR channels could be a strategy to overcome cisplatin resistance in lung cancer.

More Related Videos

Evaluating the Effectiveness of Cancer Drug Sensitization In Vitro and In Vivo09:19

Evaluating the Effectiveness of Cancer Drug Sensitization In Vitro and In Vivo

Published on: February 6, 2015

Related Concept Videos

Treatment Resistant Cancers02:56

Treatment Resistant Cancers

Cancer is the second leading cause of death in the United States. A cancer cell is genetically unstable and hence can mutate faster. They can also modify their microenvironment and escape immune surveillance. The difficulties in treating cancer are further compounded by the emergence of rapid resistance to anticancer drugs. The most common ways to attain resistance in cancer cells include alteration in drug transport and metabolism, modification of drug target, elevated DNA damage response, or...
Cystic Fibrosis: Pathogenesis01:23

Cystic Fibrosis: Pathogenesis

Cystic fibrosis (CF), an autosomal recessive disorder, significantly affects the function of exocrine glands. This genetically inherited disease is characterized by the production of thick and sticky mucus, which can severely affect various organs and systems in the body.
CF is primarily caused by a genetic mutation in a chromosome 7 gene coding for the cystic fibrosis transmembrane conductance regulator (CFTR) protein. The most common gene mutation leading to CF is the ΔF508 mutation, but...
Mechanically-gated Ion Channels01:12

Mechanically-gated Ion Channels

Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...
Voltage-gated Ion Channels01:26

Voltage-gated Ion Channels

Voltage-gated ion channels are transmembrane proteins that open and close in response to changes in the membrane potential. They are present on the membranes of all electrically excitable cells such as neurons, heart, and muscle cells.
Generally, all voltage-gated ion channels have a 'voltage-sensing domain' that spans the lipid bilayer. The charged residues in the sensor move in response to the membrane potential changes that open the channel allowing ions movement. There are several types of...