Related Experiment Video

Updated: Jun 3, 2026

Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(N-isopropylacrylamide) Microgels
11:34

Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(N-isopropylacrylamide) Microgels

Published on: September 8, 2016

Measurements of wavelength dependent scattering and backscattering coefficients by low-coherence spectroscopy

Nienke Bosschaart, Dirk J Faber, Ton G van Leeuwen

    Journal of Biomedical Optics
    |April 5, 2011
    PubMed

    More Related Videos

    Measuring Spatially- and Directionally-varying Light Scattering from Biological Material11:57

    Measuring Spatially- and Directionally-varying Light Scattering from Biological Material

    Published on: May 20, 2013

    Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy09:16

    Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

    Published on: January 9, 2017

    Related Concept Videos

    Spectrophotometry: Introduction01:16

    Spectrophotometry: Introduction

    Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
    The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
    UV–Vis Spectroscopy: Beer–Lambert Law01:09

    UV–Vis Spectroscopy: Beer–Lambert Law

    The Beer-Lambert law describes the relationship between absorbance and concentration, which combines the principles established by scientists Johann Heinrich Lambert and August Beer. Lambert's law states that when light passes through a medium, the loss in intensity is directly proportional to the original intensity and the path length of the light. Beer's law proposed that the transmittance of a solution remains constant if the product of concentration and path length is constant. The modern...
    Molecular Spectroscopy: Absorption and Emission01:14

    Molecular Spectroscopy: Absorption and Emission

    Molecules possess discrete energy levels called quantum states. Unlike atoms, which have simpler energy levels, molecules possess additional rotational and vibrational energy levels. Each energy level is separated by an energy gap, with the gaps between adjacent electronic, vibrational, and rotational levels varying significantly. The three types of energy levels in a diatomic molecule are shown in Figure 1.
    Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview01:02

    Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview

    Ultraviolet–visible (UV–visible or UV–Vis) spectroscopy is an analytical technique that investigates the interaction between matter and UV–Vis light within the electromagnetic spectrum. This method is widely used for its versatility, simplicity, and relatively quick data acquisition, making it valuable for both qualitative and quantitative analysis. When UV–Vis radiation passes through a material,  molecules absorb light depending on the energy required for electronic transitions. As a result...
    Raman Spectroscopy Instrumentation: Overview01:26

    Raman Spectroscopy Instrumentation: Overview

    A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
    The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
    Atomic Absorption Spectroscopy: Interference01:25

    Atomic Absorption Spectroscopy: Interference

    Interference leads to systematic error in atomic absorption (AA) measurements by enhancing or diminishing the analytical signal or the background. These interferences can be grouped into three main categories: spectral interference, chemical interference, and physical interference.
    Spectral interference occurs when signals from other elements or molecules overlap with the analyte signal, falsely elevating or masking the analyte's absorbance. This interference can be corrected using Zeeman,...