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Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis
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Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis

Published on: October 17, 2016

Fiber-based combined optical coherence and multiphoton endomicroscopy.

Gangjun Liu1, Zhongping Chen

  • 1University of California, Irvine, Beckman Laser Institute, Irvine, California 92612, USA.

Journal of Biomedical Optics
|April 5, 2011
PubMed
Summary

This study introduces a novel fiber-based multimodal imaging system combining multiphoton microscopy (MPM) and optical coherence microscopy (OCM). The system enables simultaneous biological sample imaging with enhanced penetration depth and efficiency.

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Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis
10:35

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High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging
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High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

Published on: January 11, 2011

Area of Science:

  • Biomedical Optics
  • Microscopy
  • Fiber Optics

Background:

  • Multimodal imaging systems offer complementary information for biological sample analysis.
  • Traditional systems often involve bulky free-space optics, limiting portability and integration.
  • Developing compact, fiber-based systems is crucial for advanced in-vivo and in-vitro imaging.

Purpose of the Study:

  • To develop and characterize a novel fiber-based multimodal imaging system.
  • To integrate multiphoton microscopy (MPM) and Fourier domain optical coherence microscopy (OCM) using a double-clad fiber (DCF) device.
  • To demonstrate simultaneous imaging capabilities in biological samples.

Main Methods:

  • A fiber-based femtosecond laser system with a 1.04 µm central wavelength was employed.
  • A single-mode fiber coupler replaced free-space optics, fused with a DCF device.
  • MPM and OCM shared the excitation light path in the DCF core; signals were collected via core and clad.

Main Results:

  • The DCF device successfully confined excitation light within the core.
  • MPM signal collection efficiency was measured at 20%, with potential for improvement.
  • Simultaneous optical coherence microscopic imaging, second harmonic generation imaging, and two-photon excitation fluorescence imaging were achieved.

Conclusions:

  • The developed fiber-based system enables efficient, simultaneous multimodal imaging.
  • The use of a DCF device offers a compact and integrated solution for combining MPM and OCM.
  • This technology holds promise for advanced biological imaging applications requiring deeper penetration and multiple contrast mechanisms.