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Design of Cecal Ligation and Puncture and Intranasal Infection Dual Model of Sepsis-Induced Immunosuppression

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Sepsis and the broken endothelium.

Nathan I Shapiro¹, William C Aird

¹Department of Emergency Medicine, Center for Vascular Biology Research, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA. nshapiro@bidmc.harvard.edu

Critical Care (London, England)

|April 5, 2011

Summary

Biomarkers soluble vascular endothelial cell growth factor receptor-1 (sVEGFR-1) and urokinase-type plasminogen activator (uPA) correlate with organ dysfunction and mortality in septic shock patients. Vascular endothelial cell growth factor (VEGF) showed no predictive value.

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Area of Science:

Critical Care Medicine
Vascular Biology
Biomarker Discovery

Background:

Sepsis is a life-threatening organ dysfunction caused by dysregulated host response to infection.
Endothelial dysfunction plays a critical role in sepsis pathophysiology.
The vascular endothelial cell growth factor (VEGF) signaling axis is implicated in sepsis, but its specific role and predictive biomarkers remain under investigation.

Purpose of the Study:

To investigate the association between circulating levels of soluble vascular endothelial cell growth factor receptor-1 (sVEGFR-1), urokinase-type plasminogen activator (uPA), and vascular endothelial cell growth factor (VEGF) with organ dysfunction and mortality in patients with septic shock due to pneumonia.

Main Methods:

The study included 81 patients with septic shock from pneumonia and 20 patients with pneumonia without organ dysfunction.
Circulating levels of sVEGFR-1, uPA, and VEGF were measured.
Statistical analyses were performed to correlate biomarker levels with clinical outcomes, including organ dysfunction and mortality.

Main Results:

Elevated circulating levels of sVEGFR-1 and uPA were significantly associated with the presence of organ dysfunction and increased mortality in septic shock patients.
Vascular endothelial cell growth factor (VEGF) levels did not show a significant association with organ dysfunction or mortality.
These findings suggest differential roles for components of the VEGF signaling axis in sepsis outcomes.

Conclusions:

sVEGFR-1 and uPA are potential diagnostic and prognostic biomarkers for organ dysfunction and mortality in septic shock.
The study reinforces the importance of endothelial activation and the VEGF signaling pathway in sepsis.
Further research into these biomarkers could lead to improved sepsis management strategies.