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Human treated dentin matrix as a natural scaffold for complete human dentin tissue regeneration
1State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, PR China.
Biomaterials
|April 5, 2011
Summary
Human treated dentin matrix (hTDM) shows potential for tooth tissue engineering. This study demonstrates hTDM effectively supports dental follicle cell growth and induces human dentin regeneration in vivo.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Regenerative Medicine
Background:
- Scaffolding materials are crucial for tooth tissue engineering.
- Rat treated dentin matrix (TDM) supports rat dentin regeneration.
- Human TDM efficacy for human dentin regeneration is unestablished.
Purpose of the Study:
- To explore the biological response to human TDM (hTDM) fabricated via a modified method.
- To assess hTDM's capacity for inducing human dentin regeneration.
Main Methods:
- Fabrication of human TDM (hTDM) using a modified dentin treatment.
- In vitro assessment of cell proliferation, migration, viability, and cytotoxicity on hTDM.
- In vivo implantation of dental follicle cells (DFCs) with hTDM in a mouse model for 8 weeks, followed by histological analysis.
Main Results:
- hTDM demonstrated improved DFC attachment, growth, viability, and reduced cytotoxicity compared to calcium phosphate controls.
- hTDM released dentinogenic factors, suggesting a role in odontogenesis.
- In vivo, hTDM induced and supported the regeneration of complete dentin tissues expressing dentin sialoprotein (DSP) and dentin matrix protein 1 (DMP-1).
Conclusions:
- Human TDM (hTDM) is a suitable biomaterial for supporting dental follicle cell activity.
- hTDM effectively induces and regenerates human dentin tissue in vivo.
- hTDM is indicated as an ideal biomaterial for human dentin regeneration applications.
