Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 3, 2026

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E (sIgE)
07:10

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E (sIgE)

Published on: April 21, 2019

Microarrayed recombinant allergens for diagnostic testing.

Wayne G Shreffler1

  • 1Food Allergy Center and Center for Immunology and Inflammatory Diseases at Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA. wshreffler@partners.org

The Journal of Allergy and Clinical Immunology
|April 5, 2011
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Strain-level microbial signatures and inferred functional alterations in infants with food protein-induced allergic proctocolitis.

Genome medicine·2026
Same author

Teff allergy in children with regular household exposure.

The journal of allergy and clinical immunology. In practice·2026
Same author

Clinical outcomes in food allergy: Implications for patients, clinicians, and health systems.

Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology·2026
Same author

Microbial metabolism of food allergens determines the severity of IgE-mediated anaphylaxis.

Cell host & microbe·2026
Same author

Management of Gastrointestinal Symptoms During Oral Immunotherapy: Guidance from OUtMATCH Investigators.

The journal of allergy and clinical immunology. In practice·2026
Same author

GPR15 and CD38 define a subset of peripheral blood pathogenic effector Th2 cells associated with active eosinophilic esophagitis.

bioRxiv : the preprint server for biology·2025
Same journal

Tamoxifen-driven neutrophil reprogramming protects from pulmonary Granulibacter bethesdensis infection in chronic granulomatous disease.

The Journal of allergy and clinical immunology·2026
Same journal

Clinical and transcriptomic characterization of mixed granulocytic COPD phenotype.

The Journal of allergy and clinical immunology·2026
Same journal

Dupilumab outcomes in pediatric asthma by early eosinophil status: post hoc analysis of VOYAGE/EXCURSION.

The Journal of allergy and clinical immunology·2026
Same journal

Maternal antibiotic exposure alters the newborn metabolomic profile and increases the risk of respiratory infections in offspring: a 13-year longitudinal birth cohort study.

The Journal of allergy and clinical immunology·2026
Same journal

Selective Elimination of Mast Cells via Siglec-6-Targeted Nanodelivery of Drug Payload.

The Journal of allergy and clinical immunology·2026
Same journal

Loss of epidermal miR-149 sensitizes to skin inflammation.

The Journal of allergy and clinical immunology·2026
See all related articles

Protein microarrays offer advanced allergy diagnosis by distinguishing clinical reactivity from sensitization. This technology shows promise, especially for food and latex allergies, improving diagnostic resolution.

Area of Science:

  • Immunology
  • Allergy Diagnostics
  • Biotechnology

Background:

  • Protein microarray-based immunoassays and recombinant allergens have co-developed over the last decade.
  • Wider application of these technologies for allergy diagnosis is accelerating.

Purpose of the Study:

  • To review the strengths and weaknesses of molecularly defined allergy testing using microarray platforms.
  • To summarize recent applications of microarray assays in allergy testing.

Main Methods:

  • Review of existing literature on protein microarrays and allergy diagnosis.
  • Analysis of microarray assay performance in various allergy contexts.
  • Summary of recent case studies and findings.

Main Results:

More Related Videos

Humanized Mediator Release Assay as a Read-Out for Allergen Potency
10:22

Humanized Mediator Release Assay as a Read-Out for Allergen Potency

Published on: June 29, 2021

Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies
10:16

Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies

Published on: September 15, 2016

Related Experiment Videos

Last Updated: Jun 3, 2026

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E (sIgE)
07:10

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E (sIgE)

Published on: April 21, 2019

Humanized Mediator Release Assay as a Read-Out for Allergen Potency
10:22

Humanized Mediator Release Assay as a Read-Out for Allergen Potency

Published on: June 29, 2021

Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies
10:16

Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies

Published on: September 15, 2016

  • Microarray platforms offer molecularly defined allergy testing with specific strengths and weaknesses.
  • Recent applications show promise, particularly for food and latex allergies.
  • The platform demonstrates potential for improved differentiation between clinical reactivity and asymptomatic sensitization.

Conclusions:

  • Protein microarrays represent a significant advancement in allergy diagnostics.
  • The technology holds potential for more precise diagnosis and management of allergic diseases.
  • Further research and application are expected to enhance clinical utility.