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Updated: Jun 3, 2026

Visualization and Quantitative Analysis of Genotoxin-Induced PARP1/PARP2 Activation in Cells Using a Fluorescent Fusion Protein-Based Reporter
Published on: April 17, 2026
[PARP inhibitors: significant progress in cancer therapy]
Françoise Dantzer1, Georges Noel, Valérie Schreiber
1UMR, BSC, CNRS-Université de Strasbourg, ESBS, poly(ADP-ribosyl)ation et intégrité du génome, Illkirch, France. francoise.dantzer@unistra.fr
Abstract:
Poly(ADP-ribosyl)ation is a post-translational modification of proteins catalyzed by poly(ADP-ribose) polymerases (PARPs). In response to genotoxic stress, PARP-1 senses DNA breaks and through the synthesis of poly(ADP-ribose) restores genome integrity by stimulating a base excision and single-strand break repair process. These properties highlight the innovative potency of PARP inhibitors to target cancer cells in their repair capacity. They open the way to promising therapeutic strategies aimed to combine PARP inhibitors with DNA-damaging chimio- or radiotherapy and as single agents for the treatment of BRCA mutation-associated tumors. The benefits to potential risks ratio of these approaches will be discussed.
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