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Quantification of Propionibacterium acidipropionici P169 bacteria in environmental samples by use of strain-specific
Min Peng1, Alexandra H Smith, Thomas G Rehberger
1Danisco Cultures Division, W227 N752 Westmound Drive, Waukesha, WI 53186, USA. min.peng@danisco.com
Applied and Environmental Microbiology
|April 5, 2011
Abstract:
A quantitative PCR (qPCR) assay targeting a gene identified by suppressive subtractive hybridization (SSH) was developed to detect Propionibacterium acidipropionici P169, with a threshold of 10(4) CFU/U of dairy feed or rumen fluid. The report is the first using a molecular marker generated by SSH to quantify a bacterial strain in environmental samples.
