Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview01:19

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview

In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then passed on to...
Development of Analytical Methods01:21

Development of Analytical Methods

An analytical methodology can be divided into four sequential steps: technique, method, procedure, and protocol. A technique is a scientific principle that rationalizes a specific phenomenon through chemical measurements. Adapting a technique for analyzing a sample of interest is termed a method. The procedure outlines the directions for performing the analysis via an analytical method. The protocol is the detailed guidelines on the procedure, which should be strictly followed to obtain the...
π Electron Effects on Chemical Shift: Overview01:27

π Electron Effects on Chemical Shift: Overview

An applied magnetic field causes loosely bound π-electrons in organic molecules to circulate, producing a local or induced diamagnetic field over a large spatial volume. As the molecules tumble in solution, the field generated by π-electrons in spherical substituents results in a zero net field. However, the net field generated by π-electrons in non-spherical substituents is not zero. The effect of this induced field depends on the orientation of the molecule with respect to B0, resulting in...
Overview of Electron Microscopy01:25

Overview of Electron Microscopy

The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
Interfacial Electrochemical Methods: Overview01:06

Interfacial Electrochemical Methods: Overview

Interfacial electrochemical methods focus on the phenomena occurring at the boundary between an electrode and a solution, as opposed to bulk methods that concentrate on the solution's overall properties. These interfacial methods are classified as either static or dynamic based on the presence of a nonzero current in the electrochemical cell and the consistency of analyte concentrations. Static methods, such as potentiometry, measure the cell's potential without any significant current passing...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Measurement of atomic scattering factors by cryoelectron microscopy.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Insights from aquaporin structures into drug-resistant sleeping sickness.

eLife·2026
Same author

Structure and activity of the essential UCH family deubiquitinase DUB16 from Leishmania donovani.

The Biochemical journal·2025
Same author

Structures of α-galactosaminidases from the CAZy GH114 family and homologs defining a new GH191 family of glycosidases.

Acta crystallographica. Section D, Structural biology·2025
Same author

Phyre2.2: A Community Resource for Template-based Protein Structure Prediction.

Journal of molecular biology·2025
Same author

Expansion of the diversity of dispersin scaffolds.

Acta crystallographica. Section D, Structural biology·2025

Related Experiment Video

Updated: Jun 3, 2026

Fully Autonomous Characterization and Data Collection from Crystals of Biological Macromolecules
07:11

Fully Autonomous Characterization and Data Collection from Crystals of Biological Macromolecules

Published on: March 22, 2019

Overview of the CCP4 suite and current developments.

Martyn D Winn1, Charles C Ballard, Kevin D Cowtan

  • 1STFC Daresbury Laboratory, Daresbury, Warrington, England. martyn.winn@stfc.ac.uk

Acta Crystallographica. Section D, Biological Crystallography
|April 5, 2011
PubMed
Summary

The CCP4 software suite aids macromolecular structure determination using X-ray crystallography. This comprehensive package offers flexible, automated pipelines for researchers in structural biology.

More Related Videos

Modeling Ligands into Maps Derived from Electron Cryomicroscopy
09:30

Modeling Ligands into Maps Derived from Electron Cryomicroscopy

Published on: July 19, 2024

Cryo-EM and Single-Particle Analysis with Scipion
09:06

Cryo-EM and Single-Particle Analysis with Scipion

Published on: May 29, 2021

Related Experiment Videos

Last Updated: Jun 3, 2026

Fully Autonomous Characterization and Data Collection from Crystals of Biological Macromolecules
07:11

Fully Autonomous Characterization and Data Collection from Crystals of Biological Macromolecules

Published on: March 22, 2019

Modeling Ligands into Maps Derived from Electron Cryomicroscopy
09:30

Modeling Ligands into Maps Derived from Electron Cryomicroscopy

Published on: July 19, 2024

Cryo-EM and Single-Particle Analysis with Scipion
09:06

Cryo-EM and Single-Particle Analysis with Scipion

Published on: May 29, 2021

Area of Science:

  • Structural Biology
  • Computational Biology
  • Biophysics

Background:

  • Macromolecular structure determination is crucial for understanding biological processes.
  • X-ray crystallography is a primary technique for solving these structures.
  • The CCP4 (Collaborative Computational Project, Number 4) suite has been a cornerstone in this field for decades.

Purpose of the Study:

  • To provide an overview of the current CCP4 software suite.
  • To highlight its flexibility and integrated infrastructure.
  • To demonstrate how individual programs contribute to a complete package for X-ray crystallography.

Main Methods:

  • The CCP4 suite integrates diverse programs via standard file formats and data objects.
  • It offers a common infrastructure and graphical interfaces for user flexibility.
  • Includes several automation pipelines for increasingly automated structure solution.

Main Results:

  • The CCP4 suite provides a comprehensive and flexible toolkit for X-ray crystallography.
  • Its integrated nature simplifies the process of macromolecular structure determination.
  • The suite supports both traditional and automated approaches to structure solution.

Conclusions:

  • The CCP4 suite remains an essential resource for the structural biology community.
  • Its continued development ensures it keeps pace with advancements in X-ray crystallography.
  • The integrated nature of CCP4 facilitates efficient and accurate structure determination.