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Related Experiment Video

Updated: Jun 3, 2026

The Automated Crystallography Pipelines at the EMBL HTX Facility in Grenoble
06:50

The Automated Crystallography Pipelines at the EMBL HTX Facility in Grenoble

Published on: June 5, 2021

Recent advances in the CRANK software suite for experimental phasing.

Navraj S Pannu1, Willem Jan Waterreus, Pavol Skubák

  • 1Biophysical Structural Chemistry, Leiden University, Leiden, The Netherlands. raj@chem.leidenuniv.nl

Acta Crystallographica. Section D, Biological Crystallography
|April 5, 2011
PubMed
Summary

CRANK software has been enhanced to automatically build more structures using anomalous diffraction and anomalous scattering data. Its improved algorithms demonstrate high performance on over 100 real datasets.

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Area of Science:

  • Crystallography
  • Structural Biology
  • Computational Biology

Background:

  • CRANK software was initially developed in 2004 for phasing anomalous scatterers using single-wavelength anomalous diffraction.
  • Subsequent development has expanded CRANK's capabilities to handle various diffraction and scattering data types.

Purpose of the Study:

  • To discuss new algorithms enhancing CRANK's structure determination capabilities.
  • To present CRANK's performance evaluation on a large number of real-world datasets.

Main Methods:

  • Implementation of novel algorithms for automated structure building.
  • Testing and validation using over 100 real crystallographic datasets.
  • Support for single-wavelength anomalous diffraction (SAD), multiple-wavelength anomalous diffraction (MAD), and single isomorphous replacement with anomalous scattering (SIRAS) data.

Main Results:

  • Significant improvements in automated structure building capabilities.
  • Demonstrated high performance and reliability across diverse datasets.
  • Expanded applicability to various anomalous diffraction and scattering techniques.

Conclusions:

  • The latest version of CRANK offers enhanced performance and broader applicability for automated structure determination.
  • CRANK is a valuable tool for researchers in structural biology and crystallography.
  • The software is freely available for download, promoting accessibility in the scientific community.