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Updated: Jun 3, 2026

Efficient iPS Cell Generation from Blood Using Episomes and HDAC Inhibitors
Published on: October 28, 2014
A more efficient method to generate integration-free human iPS cells
Keisuke Okita1, Yasuko Matsumura, Yoshiko Sato
1Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan. okita@cira.kyoto-u.ac.jp
Abstract:
We report a simple method, using p53 suppression and nontransforming L-Myc, to generate human induced pluripotent stem cells (iPSCs) with episomal plasmid vectors. We generated human iPSCs from multiple donors, including two putative human leukocyte antigen (HLA)-homozygous donors who match ∼20% of the Japanese population at major HLA loci; most iPSCs are integrated transgene-free. This method may provide iPSCs suitable for autologous and allologous stem-cell therapy in the future.

