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Related Concept Videos

Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Protein-protein Interfaces02:04

Protein-protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...

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Related Experiment Video

Updated: Jun 3, 2026

Identification of Protein Interaction Partners in Mammalian Cells Using SILAC-immunoprecipitation Quantitative Proteomics
12:53

Identification of Protein Interaction Partners in Mammalian Cells Using SILAC-immunoprecipitation Quantitative Proteomics

Published on: July 6, 2014

[Protein interactomics technologies].

A S Ivanov, V G Zgoda, A I Archakov

    Bioorganicheskaia Khimiia
    |April 5, 2011
    PubMed
    Summary

    Understanding protein-protein interactions is crucial for functional proteomics. This review compares common methods for studying these interactions in protein complexes.

    Area of Science:

    • Proteomics
    • Molecular Biology
    • Biochemistry

    Context:

    • Protein-protein interactions (PPIs) are fundamental to cellular processes.
    • Studying PPIs is essential for understanding protein function within complexes.
    • Modern proteomics increasingly relies on PPI analysis.

    Purpose:

    • To provide a concise overview of prevalent protein interactomics methods.
    • To offer a comparative evaluation of these frequently used techniques.
    • To aid researchers in selecting appropriate methods for PPI analysis.

    Summary:

    • This review details various protein interactomics approaches.
    • It evaluates common methods used for analyzing protein-protein interactions.
    • The focus is on techniques for studying proteins within stable or dynamic complexes.

    More Related Videos

    Protein Complex Affinity Capture from Cryomilled Mammalian Cells
    10:37

    Protein Complex Affinity Capture from Cryomilled Mammalian Cells

    Published on: December 9, 2016

    Related Experiment Videos

    Last Updated: Jun 3, 2026

    Identification of Protein Interaction Partners in Mammalian Cells Using SILAC-immunoprecipitation Quantitative Proteomics
    12:53

    Identification of Protein Interaction Partners in Mammalian Cells Using SILAC-immunoprecipitation Quantitative Proteomics

    Published on: July 6, 2014

    Protein Complex Affinity Capture from Cryomilled Mammalian Cells
    10:37

    Protein Complex Affinity Capture from Cryomilled Mammalian Cells

    Published on: December 9, 2016

    Impact:

    • Facilitates deeper functional insights into proteins.
    • Supports the advancement of proteomics research.
    • Aids in the selection of optimal experimental strategies for interactome mapping.