Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

Total Internal Reflection Fluorescence Microscopy

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

Phase Contrast and Differential Interference Contrast Microscopy

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Optical coherence tomography for the detection of electrothermal ureteral injury in an <i>ex-vivo</i> porcine model.

Biophotonics discovery·2026

Same author

Feasibility of non-imaging, random-sampling second harmonic generation measurements to distinguish colon cancer.

Biophotonics discovery·2026

Same author

Stalling the Enemy: Targeting Nsp13 for Next-Generation SARS-CoV-2 Antivirals.

International journal of molecular sciences·2026

Same author

Resolution cascade: simulated MTF of a coherent fiber bundle-based microendoscopic system.

Applied optics·2026

Same author

Optical Coherence Tomography Enables the Depth-Resolved Measurement of Cilia Beat Frequency in Ex Vivo Human Fallopian Tubes.

Lasers in surgery and medicine·2025

Same author

Discovery of First-in-Class Inhibitors Targeting a Pathogen-Associated Aminoglycoside-Resistance 16S rRNA Methyltransferase.

ACS infectious diseases·2025

Search research articles

Related Experiment Video

Updated: Jun 3, 2026

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Published on: February 8, 2014

Confocal-rainbow volume holographic imaging system.

Jose M Castro¹, Paul J Gelsinger-Austin, Jennifer K Barton

¹Electrical and Computer Engineering Department, University of Arizona, Tucson, Arizona 85721, USA. jmcastro0117@gmail.com

|April 5, 2011

Summary

A novel confocal-rainbow volume holographic imaging system achieves sub-16-micrometer depth resolution, overcoming limitations in broadband holographic imaging systems for enhanced 3D visualization.

More Related Videos

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

Uncovering Hidden Dynamics of Natural Photonic Structures Using Holographic Imaging

Uncovering Hidden Dynamics of Natural Photonic Structures Using Holographic Imaging

Published on: March 31, 2022

Related Experiment Videos

Last Updated: Jun 3, 2026

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Published on: February 8, 2014

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

Uncovering Hidden Dynamics of Natural Photonic Structures Using Holographic Imaging

Uncovering Hidden Dynamics of Natural Photonic Structures Using Holographic Imaging

Published on: March 31, 2022

Area of Science:

Optics and Photonics
Biomedical Imaging
Holography

Background:

Broadband volume holographic imaging systems face challenges with depth selectivity.
Resolution degradation in depth is a significant limitation for 3D imaging applications.

Purpose of the Study:

To investigate the depth selectivity performance of broadband volume holographic imaging.
To explain the mechanisms behind depth resolution degradation.
To propose and validate a novel imaging system to overcome these limitations.

Main Methods:

Theoretical modeling of holographic imaging principles.
Development of a confocal-rainbow volume holographic imaging system.
Experimental validation of the proposed system's performance.

Main Results:

The mechanism for depth resolution degradation was identified and explained.
The novel confocal-rainbow system demonstrated superior depth resolution.
Achieved depth resolution of less than 16 micrometers.
Lateral resolution of less than 2.5 micrometers within a 300x100 micrometer field of view.

Conclusions:

The confocal-rainbow volume holographic imaging system effectively overcomes depth resolution limitations.
This novel approach enables high-resolution 3D imaging with improved depth selectivity.
The system holds potential for advanced applications in microscopy and material science.