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PCR

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PCR - Polymerase Chain Reaction

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Related Experiment Video

Updated: Jun 3, 2026

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Published on: November 21, 2023

Bubble-free on-chip continuous-flow polymerase chain reaction: concept and application.

Wenming Wu¹, Kyung-Tae Kang, Nae Yoon Lee

¹Gachon BioNano Research Institute, Kyungwon University, San 65 Bokjeong-dong, Sujeong-gu, Seongnam, Gyeonggi-do 461-701, Korea.

|April 5, 2011

Summary

This study introduces a novel bubble-free sample injection method for microfluidic polymerase chain reaction (PCR) chips. The technique uses paraffin oil plugs to prevent bubble formation, ensuring reliable on-chip DNA amplification.

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Microfluidic Applications for Disposable Diagnostics

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Microfluidic Applications for Disposable Diagnostics

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Published on: February 3, 2008

Area of Science:

Microfluidics
Biotechnology
Polymerase Chain Reaction (PCR)

Background:

Bubble formation in microchannels hinders microfluidic experiments, especially for on-chip PCR.
Repetitive temperature changes in PCR exacerbate bubble formation issues.

Purpose of the Study:

To propose and validate a bubble-free sample injection scheme for continuous-flow PCR on a microfluidic chip.
To establish a theoretical foundation for bubble formation and elimination in microfluidic systems.

Main Methods:

Employing highly viscous paraffin oil plugs at both ends of the sample plug to encapsulate the sample.
Utilizing the oil plugs to increase internal channel pressure and prevent sample vaporization.
Implementing the scheme in a glass/PDMS hybrid microfluidic chip for continuous-flow PCR.

Main Results:

Demonstrated successful amplification of the D1S80 locus from clinical hair root DNA using the bubble-free injection scheme.
Confirmed chip reusability up to 10 times with consistent amplicon intensities, ensuring reliability.
Investigated the use of a cost-effective hot plate as a heating source.

Conclusions:

The proposed bubble-free sample injection scheme effectively suppresses bubble formation in microfluidic PCR.
The method ensures stable sample flow and reliable, reproducible on-chip DNA amplification.
The technique is practical for various applications, including clinical diagnostics.