Genomic characterization of two avian paramyxovirus type 2 isolates from chickens in China
Xiaojiao Li1, Shouping Zhang, Hongwei Wang
1Key Laboratory of Zoonosis of the Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, People's Republic of China.
Abstract:
The complete genome sequences were determined for avian paramyxovirus type 2 (APMV-2) strains F8 and NK isolated from chickens in China. Both strains had a genome of 14,904 nucleotides (nt) in length, which followed the "rule of six". Each genome consisted of six genes in the order 3'-N-P-M-F-HN-L-5', with a 55-nt leader at the 3' end and a 154-nt trailer at the 5' end. Sequence alignment and phylogenetic analysis showed that APMV-2 strains F8 and NK shared the highest sequence identity with APMV-2 prototype strain Yucaipa, being classified in the same subgroup as strains Yucaipa, England and Kenya, while strain Bangor represented another subgroup of APMV-2. Among the APMVs, APMV-2 strains F8 and NK exhibited a closer evolutionary relationship with APMV-7 and APMV-8 representative strains.
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