Nucleotide sequence of the phage lambda gt11 SacI-KpnI lacZ region

The nucleotide sequence of the lambda gt11 SacI-KpnI region, surrounding the unique EcoRI cloning site, was directly determined. This sequence previously had to be compiled from several diverse sources. The direct sequence confirms the sequence predicted from the compilation and pinpoints other unique restriction enzyme targets in the region for use in subcloning.