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Updated: Jun 3, 2026
Experimental Generation of Carcinoma-Associated Fibroblasts (CAFs) from Human Mammary Fibroblasts
Published on: October 25, 2011
Construction of a recombinant human FGF1 expression vector for mammary gland-specific expression in human breast
Yang Zhou1, Linzhu Ren, Jianguo Zhu
1Department of Animal Biotechnology, College of Animal Science and Veterinary Medicine, Jilin University, Changchun, 130062 Jilin, China.
Abstract:
Human Fibroblast growth factor 1 (FGF1) has been recognized as a valuable protein drug for the treatment of many diseases because of its multiple functions in regulating a variety of biological processes involved in embryonic development, cell growth and differentiation, morphogenesis, tissue repair, and others. The aim of this study was to develop an FGF1 mammary gland-specific expression vector to produce FGF1 on a large scale from transgenic cows to meet the demand for FGF1 in medical use. In this study, we generated an FGF1 mammary gland-specific expression vector and validated its function in human MCF-7 cells. This vector was shown to successfully express functional FGF1, thus potentially enabling the generation of transgenic cows to be used as mammary gland bioreactors.
