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Updated: Jun 3, 2026

An In Ovo Model for Testing Insulin-mimetic Compounds
Published on: April 23, 2018
Synthesis of a novel phosphate analog of 20-hydroxylecdysone with potent hypoglycemic activity
Dan Zhang1, Min Zhang, Bao Ding
1Department of Medicinal Natural Products and Key Laboratory of Drug Targeting & Drug Delivery Systems of the Ministry of Education, West China School of Pharmacy, Sichuan University, Chengdu, China.
Abstract:
A novel, water-soluble 20-hydroxylecdysono-20,22-phosphoric acid 2 and its sodium salt 3 were designed and synthesized from 20-hydroxylecdysone 1 in six steps and with 67% overall yield. The synthesized phosphoric acid 2 exhibited hypoglycemic activity >40-fold more potent than that of 20-hydroxylecdysone 1 at concentrations between 2 × 10⁻⁷ and 2 × 10⁻⁸ mol/l in a glucose consumption test in HepG2 cells. At a concentration of 2 × 10⁻⁹ mol/l, phosphoric acid 2 was still active, causing a maximum increase in glucose consumption of more than 500%, while 20-hydroxylecdysone 1 was inactive.
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