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Updated: Jun 3, 2026

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions
Published on: December 1, 2020
Application of 2-D DIGE to survey the quality of biological medicines
Ingrid Miller1, Manfred Gemeiner, Georg Göstl
1Department for Biomedical Sciences, Institute for Chemistry and Biochemistry, University of Veterinary Medicine Vienna, Vienna, Austria. ingrid.miller@vetmeduni.ac.at
Abstract:
2-D DIGE was used to investigate 'fingerprint proteins' in biological medicines. A presumably non-originator human albumin was analysed, and the 2-D DIGE patterns of the non-genuine and the authentic product were compared. The products could be clearly distinguished based on the pattern of minor components, which represent plasma proteins and degradation products remaining in the final products after fractionation and purification. The approach demonstrated that 2-D DIGE is an excellent tool for the analysis of biologicals of different sources and for ensuring the identity and quality of blood products.
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