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Related Experiment Videos

The gene coding for the yeast oligomycin sensitivity-conferring protein.

M Uh1, D Jones, D M Mueller

  • 1Department of Biological Chemistry and Structure, Chicago Medical School, University of Health Sciences, Illinois 60064.

The Journal of Biological Chemistry
|November 5, 1990
PubMed
Summary

The yeast ATP5 gene encodes the mitochondrial oligomycin sensitivity-conferring protein (OSCP), essential for ATP synthase function. Its transcription is repressed by glucose, impacting yeast growth on non-fermentable media.

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Area of Science:

  • Mitochondrial biology
  • Molecular genetics
  • Biochemistry

Background:

  • The oligomycin sensitivity-conferring protein (OSCP) is a subunit of the mitochondrial ATP synthase complex.
  • Understanding the gene encoding OSCP in yeast is crucial for elucidating ATP synthase assembly and function.

Purpose of the Study:

  • To sequence and characterize the gene encoding the yeast Saccharomyces cerevisiae OSCP (ATP5 gene).
  • To investigate the functional consequences of ATP5 gene disruption.
  • To analyze the transcriptional regulation of the ATP5 gene.

Main Methods:

  • Gene sequencing and characterization.
  • Construction and analysis of a null mutant strain.
  • Biochemical assays for ATPase activity.

Related Experiment Videos

  • Immunodetection of OSCP.
  • Northern blot analysis to characterize transcripts.
  • Determination of transcriptional initiation sites.

    • Main Results:

    • The ATP5 gene was sequenced, revealing homology to bovine OSCP.
    • A null mutant exhibited impaired growth on glycerol and lacked oligomycin-sensitive ATPase activity.
    • A single 1.05 kb transcript was identified, with 3-5 fold increased levels in galactose compared to glucose.
    • Transcriptional initiation occurred at two sites, independent of growth medium.

    Conclusions:

    • The ATP5 gene is essential for yeast mitochondrial ATP synthase activity and growth on non-fermentable carbon sources.
    • ATP5 gene transcription is subject to catabolite repression, indicating glucose-mediated regulation.
    • Characterization of the ATP5 gene provides insights into mitochondrial energy production regulation.