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Updated: Jun 3, 2026
Analyzing Tumor and Tissue Distribution of Target Antigen Specific Therapeutic Antibody
Published on: May 16, 2020
Preparation and characterization of monoclonal antibody against translationally controlled tumor protein
Ma Qiang1, Wu Fenfang, Geng Yan
1The Institute of Antibody Engineering, School of Biotechnology, Southern Medical University, North Guangzhou Road, Baiyun, Guangzhou, China.
Abstract:
From our previous proteomic research, we found that translationally controlled tumor protein (TCTP) might play at least a partial role in colon adenocarcinoma progression. However, the precise impact of TCTP on colorectal cancer metastasis progression is currently still unknown. Therefore, immunology reagents are urgently needed to proceed with the next mechanism-related research. Moreover, the identification of TCTP expression level in tissue of colorectal cancer patients also requires substantial amounts of immunology reagents. In this report, monoclonal antibodies (MAbs) against to TCTP were made from hyperimmune Balb/c mice, by injecting 50 μg of purified antigen intraperitoneally. Hybridomas were screened by indirect enzyme-linked immunosorbent assay (ELISA) using purified protein. Finally six mouse hybridomas producing MAbs to TCTP were established. The MAbs obtained were fully characterized using Western blot analysis and immunohistochemistry. The results showed that these antibodies could be used for the preliminary application of the next mechanism-related research and TCTP expression level analysis.
Insights
Researchers developed novel monoclonal antibodies against translationally controlled tumor protein (TCTP). These reagents are essential for studying colorectal cancer metastasis and TCTP expression levels in patients.
Area of Science:
- Oncology
- Immunology
- Molecular Biology
Background:
- Previous proteomic research suggests translationally controlled tumor protein (TCTP) involvement in colon adenocarcinoma progression.
- The specific role of TCTP in colorectal cancer metastasis remains unclear, necessitating further investigation.
- A lack of suitable immunology reagents hinders mechanistic studies and expression analysis of TCTP in colorectal cancer.
Purpose of the Study:
- To generate and characterize monoclonal antibodies (MAbs) against TCTP.
- To provide essential immunology reagents for colorectal cancer research.
- To facilitate the study of TCTP's role in colorectal cancer metastasis and expression analysis.
Main Methods:
- Monoclonal antibodies against TCTP were produced in hyperimmune Balb/c mice via intraperitoneal injection of purified antigen.
- Hybridomas were screened using indirect enzyme-linked immunosorbent assay (ELISA) with purified TCTP.
- Established MAbs were characterized by Western blot analysis and immunohistochemistry.
Main Results:
- Six mouse hybridomas producing MAbs specific to TCTP were successfully established.
- Characterization confirmed the specificity and utility of the generated MAbs.
- The antibodies demonstrated potential for preliminary application in mechanism-related research and TCTP expression analysis.
Conclusions:
- Novel monoclonal antibodies against TCTP have been developed.
- These antibodies are suitable for preliminary research into TCTP's role in colorectal cancer.
- The generated reagents will support further investigation of TCTP in colorectal cancer progression and metastasis.
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