Collection and handling of blood specimens for peptidomics

Pre-analytical variables can alter the analysis of blood-derived samples. In particular, sample collection and specimen preparation can alter the validity of results obtained by modern multiplex assays (e.g., LC-MS). Low-molecular weight proteins (peptides) as products of proteolytic cleavage events exhibit a close connection to protease activity and function. Altered proteolytic activity during sample collection, preparation, handling, and storage is mirrored by alterations in abundance of specific peptides. Awareness of clinical practices in medical laboratories allows for the identification of specific variables that may affect the results of a peptidomic study. Knowledge of pre-analytical variables is a prerequisite to understand and control their impact.