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Updated: Jun 3, 2026
06:59
Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile
Published on: June 13, 2016
Exosome isolation for proteomic analyses and RNA profiling
Douglas D Taylor1, Wolfgang Zacharias, Cicek Gercel-Taylor
1James Graham Brown Cancer Center, University of Louisville School of Medicine, Louisville, KY, USA. ddtaylor@louisville.edu
Methods in Molecular Biology (Clifton, N.J.)
|April 7, 2011
Summary
Exosomes, tiny vesicles released by tumors, carry diagnostic microRNAs (miRNAs) and proteins. ExoQuick precipitation yields purer, more abundant exosomal RNA and protein than other methods, improving cancer detection accuracy.
Area of Science:
- Biochemistry
- Molecular Biology
- Oncology
Background:
- Exosomes, released by tumor cells, contain proteins and microRNAs (miRNAs) with diagnostic potential.
- Circulating exosomal content can serve as a non-invasive biomarker for cancer detection.
Purpose of the Study:
- To compare different methods for purifying exosomes for downstream molecular analysis.
- To assess the quantity and quality of RNA and protein isolated from exosomes using various purification techniques.
Main Methods:
- Exosomes were isolated using ExoQuick precipitation, chromatography, ultracentrifugation, and DynaBeads.
- Isolated exosomes were analyzed for RNA and protein quantity and quality.
Main Results:
- ExoQuick precipitation yielded higher purity and quantity of exosomal RNA and protein compared to other methods.
- While not cell-specific, ExoQuick enhances downstream analyses like miRNA profiling and protein analysis.
Conclusions:
- ExoQuick precipitation is a superior method for isolating exosomes for diagnostic biomarker analysis.
- Improved exosome purification enhances the sensitivity and accuracy of cancer detection assays.
Related Concept Videos
01:36Overview of Exosomes
Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
02:24Ribosome Profiling
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...