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Updated: Jun 3, 2026

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Establishment of Cancer Stem Cell Cultures from Human Conventional Osteosarcoma
Published on: October 14, 2016
WST-8 analysis of cell viability during osteogenesis of human mesenchymal stem cells
1AO Research Institute Davos, Davos Platz, Switzerland. martin.stoddart@aofoundation.org
Methods in Molecular Biology (Clifton, N.J.)
|April 7, 2011
Abstract:
WST-8 is one of the newer generation formazan-based dyes, which release the converted product into the medium in a soluble form. This allows for a non-destructive determination of viability enabling the cells to be subject to further investigations. This is a major advantage in cases where cell phenotype is being investigated and data such as matrix synthesis is correlated to cell number. This chapter describes the use of WST-8 to normalise alkaline phosphatase activity.
