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An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects
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Raman micro-spectroscopy as a non-invasive cell viability test.

Sophie Verrier1, Alina Zoladek, Ioan Notingher

  • 1AO Research Institute, Davos Platz, Switzerland. sophie.verrier@aofoundation.org

Methods in Molecular Biology (Clifton, N.J.)
|April 7, 2011
PubMed
Summary

Raman spectroscopy offers a non-invasive method to monitor cell health. This technique can differentiate between living and dead cells, and cells in various cell cycle stages, aiding toxicology and tissue engineering.

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Spectroscopy

Background:

  • Assessing cell viability is crucial in biological research, toxicology, and tissue engineering.
  • Current methods for cell death analysis are often invasive, requiring cell fixation or extraction.
  • There is a need for non-invasive techniques to monitor cell health in real-time.

Purpose of the Study:

  • To demonstrate the utility of Raman spectroscopy as a non-invasive method for cell analysis.
  • To show Raman spectroscopy's capability in distinguishing cells at different cell cycle stages.
  • To validate Raman spectroscopy for differentiating living from dead cells.

Main Methods:

  • Utilizing Raman spectroscopy for label-free, non-invasive analysis of living cells.
  • Applying the technique to distinguish cells in different cell cycle phases.

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Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
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Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

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Viability Assays for Cells in Culture
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Published on: January 20, 2014

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An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects
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Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
13:48

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Published on: May 29, 2012

Viability Assays for Cells in Culture
12:03

Viability Assays for Cells in Culture

Published on: January 20, 2014

  • Employing Raman spectroscopy to assess cell viability by differentiating live and dead cells.

    • Main Results:

    • Raman spectroscopy successfully distinguished cells at various cell cycle stages.
    • The technique effectively differentiated between living and dead cells.
    • Two examples demonstrated the performance and applicability of Raman spectroscopy for cell viability assessment.

    Conclusions:

    • Raman spectroscopy is a powerful non-invasive tool for cell biology.
    • This method offers a valuable alternative to invasive techniques for cell health monitoring.
    • Raman spectroscopy has significant potential in fields like toxicology and tissue engineering.