Related Experiment Video
Updated: Jun 3, 2026

Measurement of Specific Mycobacterial Mistranslation Rates with Gain-of-function Reporter Systems
Published on: April 26, 2019
The wag31 gene of Mycobacterium tuberculosis is positively regulated by the stringent response
John L Dahl1, Dalia Lau Bonilla
1Department of Biology, University of Minnesota Duluth, Duluth, MN, USA. jldahl@d.umn.edu
Abstract:
The stringent response of Mycobacterium tuberculosis is coordinated by Rel and is required for full virulence in animal models. A serological-based approach identified Wag31(Mtb) as a protein that is upregulated in M. tuberculosis in a rel-dependent manner. This positive regulation was confirmed by analysis of M. tuberculosis mRNA expression. Mycobacterium smegmatis was used to confirm that the expression of wag31(Mtb) from its native promoter is positively regulated by the stringent response. Furthermore, elevated wag31(Mtb) expression in M. smegmatis drastically alters the cell-surface hydrophobic properties.
More Related Videos
Related Concept Videos
Stringent Response in E. coli
Regulation of Bacterial Virulence
Global Regulatory Systems
Other Stress Responses in Bacteria
Translational Regulation
Prokaryotic Transcriptional Activators and Repressors
Transcription of prokaryotic...

