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Identification and purification of calpactins from cardiac muscle and their effect on Na+/Ca2+ exchange activity
A Sobota1, F Cusinato, S Luciani
1Department of Pharmacology, University of Padova, Italy.
Biochemical and Biophysical Research Communications
|November 15, 1990
Abstract:
Calpactins were purified from bovine cardiac muscle by a slightly modified Glenney et al. procedure (J. Cell. Biol. 104, 503-511, 1987). Two major proteins (apparent MW of 36 and 68 kDa) able to bind phospholipids in a Ca2(+)-dependent manner were identified. These proteins completely reversed the inhibition of Na+/Ca2+ exchange activity of cardiac sarcolemmal vesicles consequent to EGTA-treatment. A modulation of cardiac Na+/Ca2+ exchange activity by calpactins is suggested.