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Cytomegalovirus Disease

Cytomegalovirus Disease

Cytomegalovirus (CMV) disease is caused by human cytomegalovirus, a double-stranded DNA virus of the Herpesviridae family. While primary CMV infection is often asymptomatic in immunocompetent individuals, the virus can cause severe disease in neonates and immunocompromised patients. CMV is the most common cause of congenital viral infection in the United States, and a major pathogen in solid organ and hematopoietic stem cell transplant recipients.CMV is transmitted via bodily fluids, sexual...

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Updated: Jun 3, 2026

Use of In vivo Imaging to Monitor the Progression of Experimental Mouse Cytomegalovirus Infection in Neonates

Use of In vivo Imaging to Monitor the Progression of Experimental Mouse Cytomegalovirus Infection in Neonates

Published on: July 6, 2013

Temporal profiling of the coding and noncoding murine cytomegalovirus transcriptomes.

Paul Lacaze¹, Thorsten Forster, Alan Ross

¹Division of Pathway Medicine, University of Edinburgh Medical School, The Chancellor's Building, 49 Little France Crescent, Edinburgh EH16 4SB, United Kingdom.

Journal of Virology

|April 8, 2011

Summary

This summary is machine-generated.

Murine cytomegalovirus (MCMV) gene expression programming is primarily dependent on the ie3 gene, not ie1 or ie2. This study reveals genome-wide noncoding and bidirectional transcription during MCMV infection.

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Area of Science:

Virology
Molecular Biology
Genomics

Background:

The complete transcriptional landscape of murine cytomegalovirus (MCMV), including coding, noncoding, and antisense RNA, is not fully understood.
Investigating MCMV transcription is crucial for understanding viral replication and pathogenesis.

Purpose of the Study:

To develop a genome-wide platform for analyzing MCMV coding and noncoding transcription.
To elucidate the viral transcriptional program and identify key regulatory factors during MCMV infection.

Main Methods:

Development of a custom oligonucleotide microarray for genome-wide transcription profiling.
Profiling of MCMV wild-type and immediate-early mutant strains in fibroblast cells.
Analysis of transcriptional activation at 6.5 hours post-infection.

Main Results:

A rapid activation of the MCMV transcriptome was observed by 6.5 hours post-infection.
Genomic programming of viral gene expression demonstrated an absolute dependency on the ie3 gene, with no reliance on ie1 or ie2.
Genome-wide noncoding and bidirectional transcription were identified for the first time during late stages of MCMV infection.

Conclusions:

The immediate-early 3 (ie3) gene is essential for the genomic programming of MCMV gene expression.
This study provides novel insights into the complex transcriptional dynamics of MCMV, including previously uncharacterized noncoding and bidirectional transcription.