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Expression of CD45 isoforms in lymph node reactive hyperplasia.

C W Caldwell1

  • 1Department of Pathology, University of Missouri School of Medicine, Columbia 65212.

Clinical Immunology and Immunopathology
|December 1, 1990
PubMed
Summary
This summary is machine-generated.

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T cells switch from CD45RA to CD45RO isoforms upon activation, indicating differentiation. This CD45 isoform switch, observed in human hyperplastic lymph nodes, signifies memory or late T cell activation.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • The CD45 antigen family comprises multiple molecular isoforms (180-220 kDa).
  • CD45RA recognizes high molecular weight isoforms, associated with naive T cells.
  • CD45RO recognizes low molecular weight isoforms, associated with memory T cells.

Purpose of the Study:

  • To investigate the in vivo CD45 isoform expression in human hyperplastic lymph nodes.
  • To confirm the in vitro observed T cell CD45 isoform switch in an in vivo setting.

Main Methods:

  • Analysis of CD45 isoform expression (CD45RA and CD45RO) on T cells.
  • Comparison of T cells from hyperplastic versus nonhyperplastic human lymph nodes.

Main Results:

  • Hyperplastic lymph nodes showed an in vivo T cell CD45 isoform switch from CD45RA+ to CD45RO+.

Related Experiment Videos

  • The percentage of CD45RO+ T cells did not correlate with other lymphoid activation parameters.

    • Conclusions:

    • CD45RO expression in vivo likely marks T cell differentiation and acquisition of memory.
    • The observed in vivo switch supports the role of CD45 isoforms as markers of T cell activation states.