Related Experiment Video
Updated: Jun 3, 2026
A Microplate Assay to Assess Chemical Effects on RBL-2H3 Mast Cell Degranulation: Effects of Triclosan without Use of an Organic Solvent
Published on: November 1, 2013
In vitro inhibitory effect of trichostatin A on canine grade 3 mast cell tumor
Marcia Kazumi Nagamine1, Daniel S Sanches, Katia C Pinello
1Laboratory of Experimental Oncology, Department of Pathology, School of Veterinary Medicine and Animal Science, University of São Paulo, SP, Brazil.
Abstract:
Mast cell tumor (MCT) is one of the most prevalent neoplasms that affect skin and soft tissue in dogs. Because mast cell tumors present a great variety of clinical appearance and behavior, their treatment becomes a challenge. Trichostatin A (TSA), an antifungal antibiotic, has shown inhibitory effects on the proliferation and induction of apoptosis in various types of cancer cells. In order to evaluate the potential of trichostatin A as a therapeutic drug, cells of grade 3 MCT were cultured and treated with concentrations of 1 nM to 400 nM of TSA. MTT assay and trypan blue exclusion assays were performed to estimate cell growth and cell viability, and cell cycle analysis was evaluated. TSA treatment showed a reduction in numbers of viable cells and an increase of cell death by apoptosis. The cell cycle analysis showed an increase of hypodiploid cells and a reduction of G0/G1 and G2/M -phases. According to these results, trichostatin A may be an interesting potential chemotherapeutic agent for the treatment of canine MCT.
Insights
Trichostatin A (TSA) effectively reduced viable canine mast cell tumor (MCT) cells and induced apoptosis. This study suggests TSA shows potential as a chemotherapeutic agent for treating canine MCT.
Area of Science:
- Veterinary Oncology
- Cancer Biology
- Pharmacology
Background:
- Canine mast cell tumors (MCTs) are common skin neoplasms with variable clinical presentations, posing treatment challenges.
- Trichostatin A (TSA), an antifungal antibiotic, exhibits anti-cancer properties, including proliferation inhibition and apoptosis induction in various cancer types.
Purpose of the Study:
- To evaluate the therapeutic potential of Trichostatin A (TSA) against canine mast cell tumors (MCTs).
Main Methods:
- Grade 3 canine MCT cells were cultured and treated with varying concentrations of TSA (1 nM to 400 nM).
- Cell viability and proliferation were assessed using MTT and trypan blue exclusion assays.
- Cell cycle analysis was performed to evaluate TSA's effects on cell cycle progression.
Main Results:
- TSA treatment significantly reduced the number of viable MCT cells.
- TSA induced cell death through apoptosis.
- Cell cycle analysis revealed an increase in hypodiploid cells and a decrease in G0/G1 and G2/M phases.
Conclusions:
- Trichostatin A demonstrates significant anti-proliferative and pro-apoptotic effects on canine mast cell tumor cells in vitro.
- TSA presents a promising potential as a chemotherapeutic agent for treating canine MCTs.
