Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multiparametric profiling of non-small-cell lung cancers reveals distinct immunophenotypes.

JCI insight·2016
Same author

An Integrative Analysis of the InR/PI3K/Akt Network Identifies the Dynamic Response to Insulin Signaling.

Cell reports·2016
Same author

Adaptive resistance to therapeutic PD-1 blockade is associated with upregulation of alternative immune checkpoints.

Nature communications·2016
Same author

Evidence of off-target effects associated with long dsRNAs in Drosophila melanogaster cell-based assays.

Nature methods·2006
Same author

Super-size flies.

Cell metabolism·2005
Same author

cis-regulatory logic of short-range transcriptional repression in Drosophila melanogaster.

Molecular and cellular biology·2005
Same journal

Nondenaturing Polyacrylamide Gel Electrophoresis: Preparation and Analysis of DNA.

Current protocols in molecular biology·2021
Same journal

Purification and Concentration of DNA from Aqueous Solutions: Preparation and Analysis of DNA.

Current protocols in molecular biology·2021
Same journal

Expression of Proteins Using Semliki Forest Virus Vectors: Protein Expression.

Current protocols in molecular biology·2021
Same journal

Methylation and Uracil Interference Assays for Analysis of Protein-DNA Interactions: DNA-Protein Interactions.

Current protocols in molecular biology·2021
Same journal

Separation of Double- and Single-Stranded Nucleic Acids Using Hydroxylapatite Chromatography: Preparation and Analysis of DNA.

Current protocols in molecular biology·2021
Same journal

Pulsed-Field Gel Electrophoresis: Preparation and Analysis of DNA.

Current protocols in molecular biology·2021
See all related articles

Related Experiment Video

Updated: Jun 3, 2026

Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform
09:13

Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform

Published on: January 13, 2016

Digital multiplexed gene expression analysis using the NanoString nCounter system.

Meghana M Kulkarni1

  • 1Harvard Medical School, Boston, Massachusetts, USA.

Current Protocols in Molecular Biology
|April 8, 2011
PubMed
Summary
This summary is machine-generated.

The NanoString nCounter Gene Expression Assay offers a sensitive method for detecting up to 800 gene expressions. This digital detection assay bypasses reverse transcription and PCR amplification for accurate mRNA quantification.

More Related Videos

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform
10:37

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

Published on: November 30, 2016

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
07:27

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Related Experiment Videos

Last Updated: Jun 3, 2026

Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform
09:13

Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform

Published on: January 13, 2016

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform
10:37

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

Published on: November 30, 2016

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
07:27

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • Gene expression profiling is crucial for understanding biological processes.
  • Existing methods like microarrays and qPCR have limitations in throughput, sensitivity, or complexity.
  • A need exists for a method bridging genome-wide and targeted expression analysis.

Purpose of the Study:

  • To present the protocol for the NanoString nCounter Gene Expression Assay.
  • To highlight its robustness, reproducibility, sensitivity, and linearity.
  • To detail its application in quantifying gene expression.

Main Methods:

  • Direct digital detection of mRNA using target-specific, color-coded probe pairs.
  • Utilizes reporter probes with unique color codes and capture probes with biotin labels.
  • Involves solution-phase hybridization, immobilization in an nCounter cartridge, and digital analysis.
  • Bypasses the need for reverse transcription and PCR amplification.

Main Results:

  • The assay can detect up to 800 genes in a single reaction.
  • Achieves high sensitivity and linearity across a broad range of expression levels.
  • Provides direct digital counts of molecular barcodes for accurate gene expression measurement.

Conclusions:

  • The NanoString nCounter assay is a powerful tool for gene expression profiling.
  • It offers a robust, sensitive, and reproducible alternative to traditional methods.
  • Enables precise quantification of mRNA molecules without amplification steps.