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Updated: Jun 3, 2026

Measurement of Chitinase Activity in Biological Samples
Published on: August 22, 2019
[Chitinolytic activity of bacteria]
Elzbieta Saks1, Urszula Jankiewicz
1Katedra Biochemii, Wydział Rolnictwa i Biologii Szkoły Głównej Gospodarstwa Wiejskiego, Warszawa.
Abstract:
Chitinolytic bacteria play an important role in degradation of chitin, one of the most abundant biopolymers in nature. These microorganisms synthesize specific enzymes, that catalyze hydrolysis of beta-1,4-glycosidic bonds in low-digestible chitin polymers, turning it into low-molecular, easy to digest compounds. During last decades many bacterial chitinolytic enzymes have been studied and characterized, mainly for their potential applications in agriculture, industry and medicine. Several chitinase classifications have been proposed, either on the base of substrate specificity or amino acid sequence similarities. X-ray crystallography and NMR spectroscopy techniques enabled the determination of three dimensional structure of some chitinases, what was helpful in explaining their catalytic mechanism. Development of biotechnology and molecular biology enables a deep research in regulation and cloning of bacterial chitinase genes.
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