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Related Concept Videos

Voltammetric Techniques: Cyclic Voltammetry01:10

Voltammetric Techniques: Cyclic Voltammetry

Cyclic voltammetry (CV) is an electrochemical technique used to investigate the redox properties of a chemical species. It involves measuring the current response of an electrochemical cell as a function of the applied potential. The setup for cyclic voltammetry typically consists of a working electrode, a reference electrode, and a counter electrode—all immersed in an electrolyte solution. The working electrode is where the redox reaction of interest occurs, while the reference electrode...
Voltammetric Techniques: Linear-Scan (E vs Time)01:12

Voltammetric Techniques: Linear-Scan (E vs Time)

Polarography is a classical voltammetric technique used to analyze electrochemical reactions. This method applies a linear potential sweep to a dropping mercury electrode (DME), and the resulting current is measured. A dropping mercury electrode is commonly used as the working electrode in polarography. It consists of a capillary tube filled with mercury, where the tiny droplet forms at the tip. This droplet continuously drops from the capillary, creating a new electrode surface for each...
Voltammetric Techniques: Pulse Voltammetry01:17

Voltammetric Techniques: Pulse Voltammetry

Differential-pulse voltammetry (DPV) is a type of voltammetry that involves applying a series of voltage pulses to an electrochemical cell while measuring the resulting current. In DPV, the differential pulse or small potential pulses are superimposed on a linear potential sweep. The magnitude of these pulses is typically small, often in the millivolt range. Each voltage pulse lasts a short duration, usually in the order of a few milliseconds, and is applied at regular intervals along the...
Voltammetry: Overview01:20

Voltammetry: Overview

Voltammetry is an electroanalytical technique in which the current flowing through an electrochemical cell is measured as a function of applied potential, typically under conditions of concentration polarization. The technique provides valuable information about redox-active species, and the current response is plotted as a voltammogram.
A voltammetric cell uses three electrodes: a working electrode, a reference electrode, and an auxiliary electrode. The redox reactions occur in the working...
Voltammetry: Stripping Methods01:13

Voltammetry: Stripping Methods

Anodic Stripping Voltammetry (ASV), Cathodic Stripping Voltammetry (CSV), and Adsorptive Stripping Voltammetry (AdSV) are electrochemical techniques used to determine trace amounts of analytes in solution. These methods involve applying a potential to an electrode and measuring the resulting current.
Anodic Stripping Voltammetry (ASV)
ASV is used to determine metals and metalloids at trace levels. It involves two steps: deposition and stripping. First, a negative potential is applied to the...
Voltammetry: Factors Affecting Measurements01:21

Voltammetry: Factors Affecting Measurements

A current produced due to the redox reactions of the analyte at the working and auxiliary electrodes is called a faradaic current. The reaction can be divided into two types. The current generated due to the reduction of the analyte is called cathodic current, and it carries a positive charge. In contrast, the current produced by analyte oxidation is known as an anodic current, and it has a negative charge. The applied potential at the working electrode determines the faradaic current flow, and...

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Related Experiment Video

Updated: Jun 3, 2026

Combined Infusion and Stimulation with Fast-Scan Cyclic Voltammetry (CIS-FSCV) to Assess Ventral Tegmental Area Receptor Regulation of Phasic Dopamine
06:40

Combined Infusion and Stimulation with Fast-Scan Cyclic Voltammetry (CIS-FSCV) to Assess Ventral Tegmental Area Receptor Regulation of Phasic Dopamine

Published on: April 23, 2020

Higher sensitivity dopamine measurements with faster-scan cyclic voltammetry.

Richard B Keithley1, Pavel Takmakov, Elizabeth S Bucher

  • 1Department of Chemistry, University of North Carolina at Chapel Hill, 27599, United States.

Analytical Chemistry
|April 9, 2011
PubMed
Summary

Fast-scan cyclic voltammetry (FSCV) using a 1.0 V waveform with increased scan rates improved dopamine detection sensitivity and speed. A novel 1.3 V sawhorse waveform further enhanced sensitivity and stability for in vivo neurotransmitter monitoring.

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Presynaptic Dopamine Dynamics in Striatal Brain Slices with Fast-scan Cyclic Voltammetry
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Presynaptic Dopamine Dynamics in Striatal Brain Slices with Fast-scan Cyclic Voltammetry

Published on: January 12, 2012

Related Experiment Videos

Last Updated: Jun 3, 2026

Combined Infusion and Stimulation with Fast-Scan Cyclic Voltammetry (CIS-FSCV) to Assess Ventral Tegmental Area Receptor Regulation of Phasic Dopamine
06:40

Combined Infusion and Stimulation with Fast-Scan Cyclic Voltammetry (CIS-FSCV) to Assess Ventral Tegmental Area Receptor Regulation of Phasic Dopamine

Published on: April 23, 2020

Presynaptic Dopamine Dynamics in Striatal Brain Slices with Fast-scan Cyclic Voltammetry
08:49

Presynaptic Dopamine Dynamics in Striatal Brain Slices with Fast-scan Cyclic Voltammetry

Published on: January 12, 2012

Area of Science:

  • Neuroscience
  • Analytical Chemistry
  • Electrochemistry

Background:

  • Fast-scan cyclic voltammetry (FSCV) with carbon-fiber microelectrodes detects in vivo catecholamine release.
  • Standard waveforms (1.0 V or 1.3 V) present trade-offs between temporal resolution and sensitivity.

Purpose of the Study:

  • To enhance the sensitivity of the 1.0 V FSCV waveform while maintaining rapid temporal response.
  • To develop a more stable and sensitive waveform for in vivo dopamine detection.

Main Methods:

  • Increased scan rate for the 1.0 V waveform up to 2400 V/s.
  • Implemented analog background subtraction to manage high currents.
  • Adapted the 1.3 V triangular waveform into a sawhorse design.

Main Results:

  • The 1.0 V waveform with increased scan rate yielded a 4-fold increase in signal-to-noise ratio in vitro and in vivo.
  • The 1.3 V sawhorse waveform reduced dopamine detection limits to 0.96 ± 0.08 nM in vitro and improved in vivo performance.
  • Electron microscopy indicated dopamine sensitivity is in a quasi-steady state at potentials above 1.0 V.

Conclusions:

  • Increasing scan rate is an effective strategy to improve FSCV sensitivity and temporal resolution.
  • The 1.3 V sawhorse waveform offers a significant advancement for sensitive and stable in vivo dopamine monitoring.
  • These waveform modifications enhance FSCV's utility for studying neurochemical events.