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Related Concept Videos

Hybridoma Technology01:31

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...
B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
Humoral Immune Responses01:36

Humoral Immune Responses

Overview
Special Features of Adaptive Immunity01:20

Special Features of Adaptive Immunity

The adaptive immune system, a crucial component of the overall immune response, offers a highly specialized defense against pathogens. It involves specific cell types and features, enabling it to combat infections effectively and efficiently.
The primary cell types involved in adaptive immunity are T cells and B cells. Each type has a unique role in defending the body against pathogens. T cells are responsible for cell-mediated immunity. They identify and eliminate infected cells directly,...
Antibody Actions01:26

Antibody Actions

Antibodies, or immunoglobulins, are critical players in the immune system's arsenal against invading pathogens. Produced by B cells and plasma cells, their primary role is to detect and bind to specific antigens, molecules found on the surface of pathogens like bacteria or viruses. Beyond antigen recognition, antibodies perform several vital functions that contribute to immune defense.
Neutralization
Antibodies can bind to pathogens, preventing them from infecting host cells. This process...

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Related Experiment Video

Updated: Jun 3, 2026

Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations
09:07

Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations

Published on: August 22, 2019

Single B cell antibody technologies.

Thomas Tiller1

  • 1Max Planck Institute for Infection Biology, D-10117 Berlin, Germany. thomas.tiller@morphosys.com

New Biotechnology
|April 9, 2011
PubMed
Summary
This summary is machine-generated.

Monoclonal antibodies (mAbs) are vital biologics. This review explores single B cell methods for discovering therapeutic mAbs and advancing research, highlighting key isolation and cloning techniques.

More Related Videos

The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of Humoral Immunity
08:26

The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of Humoral Immunity

Published on: December 14, 2016

Related Experiment Videos

Last Updated: Jun 3, 2026

Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations
09:07

Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations

Published on: August 22, 2019

The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of Humoral Immunity
08:26

The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of Humoral Immunity

Published on: December 14, 2016

Area of Science:

  • Biotechnology
  • Immunology
  • Pharmacology

Background:

  • Monoclonal antibodies (mAbs) represent a critical class of biologics.
  • mAbs are extensively utilized in pharmaceutical and diagnostic applications.
  • Existing technologies focus on mAb generation and identification for therapeutic relevance.

Purpose of the Study:

  • To review diverse single B cell approaches.
  • To describe the application of these methods in discovering therapeutic mAbs.
  • To highlight their utility in basic scientific research.

Main Methods:

  • Isolation of immunoglobulin (Ig) encoding genes from single B-lineage cells.
  • Cloning of Ig genes from single B-lineage cells.
  • Review of various single B cell isolation and cloning technologies.

Main Results:

  • Summary of multiple single B cell strategies.
  • Description of how these methods facilitate mAb discovery.
  • Identification of mAbs with therapeutic potential.

Conclusions:

  • Single B cell technologies are instrumental for mAb discovery.
  • These methods support both therapeutic development and fundamental research.
  • The review provides insights into optimizing mAb generation through single cell techniques.