Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Preparation of Samples for Electron Microscopy

Preparation of Samples for Electron Microscopy

To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Lung infection of avian pathogenic Escherichia coli co-upregulates the expression of cSP-A and cLL in chickens.

Research in veterinary science·2022

Same author

Nickel/nitrogen-doped carbon nanocomposites: Synthesis and electrochemical sensor for determination of p-nitrophenol in local environment.

Environmental research·2022

Same author

Ca<sup>2+</sup> homeostasis maintained by TMCO1 underlies corpus callosum development via ERK signaling.

Cell death & disease·2022

Same author

Potato (Solanum tuberosum L.) non-specific lipid transfer protein StLTP6 promotes viral infection by inhibiting virus-induced RNA silencing.

Planta·2022

Same author

Prevalence and associated factors of frailty among community dwelling older adults in Northwest China: a cross-sectional study.

BMJ open·2022

Same author

Identifying the Subtypes of Major Depressive Disorder Based on Somatic Symptoms: A Longitudinal Study Using Latent Profile Analysis.

Frontiers in psychiatry·2022

Search research articles

Related Experiment Video

Updated: Jun 3, 2026

Advanced Experimental Methods for Low-temperature Magnetotransport Measurement of Novel Materials

Advanced Experimental Methods for Low-temperature Magnetotransport Measurement of Novel Materials

Published on: January 21, 2016

Magnetron sputtering Si interlayer: a protocol to prepare solid phase microextraction coatings on metal-based fiber.

Hongmei Liu¹, Li Ji, Jubai Li

¹Key Laboratory of Chemistry of Northwestern Plant Resources, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, China.

Journal of Chromatography. A

|April 9, 2011

Summary

This study developed a novel method for creating durable metal-based solid phase microextraction (SPME) fibers. The new technique uses a silicon interlayer for robust multiwalled carbon nanotube coatings, enhancing fiber longevity and stability.

More Related Videos

Metal-Assisted Electrochemical Nanoimprinting of Porous and Solid Silicon Wafers

Metal-Assisted Electrochemical Nanoimprinting of Porous and Solid Silicon Wafers

Published on: February 8, 2022

Sputter Growth and Characterization of Metamagnetic B2-ordered FeRh Epilayers

Sputter Growth and Characterization of Metamagnetic B2-ordered FeRh Epilayers

Published on: October 5, 2013

Related Experiment Videos

Last Updated: Jun 3, 2026

Advanced Experimental Methods for Low-temperature Magnetotransport Measurement of Novel Materials

Advanced Experimental Methods for Low-temperature Magnetotransport Measurement of Novel Materials

Published on: January 21, 2016

Metal-Assisted Electrochemical Nanoimprinting of Porous and Solid Silicon Wafers

Metal-Assisted Electrochemical Nanoimprinting of Porous and Solid Silicon Wafers

Published on: February 8, 2022

Sputter Growth and Characterization of Metamagnetic B2-ordered FeRh Epilayers

Sputter Growth and Characterization of Metamagnetic B2-ordered FeRh Epilayers

Published on: October 5, 2013

Area of Science:

Analytical Chemistry
Materials Science
Nanotechnology

Background:

Conventional fused-silica solid phase microextraction (SPME) fibers suffer from fragility.
Surface modification of metal substrates for SPME is challenging, limiting traditional coating techniques.

Purpose of the Study:

To develop a robust surface modification protocol for metal-based SPME fibers.
To enable the application of mature coating techniques on metal substrates for enhanced SPME performance.

Main Methods:

Magnetron sputtering of a silicon (Si) interlayer onto a stainless steel fiber.
Modification of the Si interlayer with active groups.
Attachment of a multiwalled carbon nanotubes (MWCNTs) coating onto the Si interlayer.

Main Results:

The developed protocol successfully created a firm Si interlayer on stainless steel.
The MWCNTs coating adhered well to the Si interlayer, preserving SPME performance.
The resulting MWCNTs/Si/stainless steel fibers demonstrated high rigidity, extended service life, and excellent stability in harsh conditions (high temperature, acid, alkali).

Conclusions:

The magnetron sputtering of a Si interlayer provides a versatile solution for coating unconfined substrates.
This technique overcomes the limitations of traditional methods for metal-based SPME fiber fabrication.
The novel metal-based SPME fibers offer significant advantages in durability and stability for analytical applications.