Related Experiment Video

Updated: Jun 2, 2026

Generation of Myospheres From hESCs by Epigenetic Reprogramming
09:32

Generation of Myospheres From hESCs by Epigenetic Reprogramming

Published on: June 21, 2014

Impact of SOCS3 overexpression on human skeletal muscle development in vitro

Marissa K Caldow1, Gregory R Steinberg, David Cameron-Smith

  • 1Molecular Nutrition Unit, School of Exercise and Nutrition Sciences, Deakin University, Burwood, Victoria, Australia. marissa.caldow@deakin.edu.au

Cytokine
|April 12, 2011
PubMed

Insights

Suppressors of cytokine signaling 3 (SOCS3) enhances muscle cell differentiation and hypertrophy by upregulating STAT3 target genes, independent of STAT3

Area of Science:

  • Muscle regeneration and development
  • Cell signaling pathways
  • Molecular biology

Background:

  • The Janus kinase/signal transducers and activators of transcription (JAK/STAT) pathway is critical for myogenesis.
  • STAT3 is essential for satellite cell migration and myogenic differentiation.
  • Suppressors of cytokine signaling (SOCS) proteins regulate STAT activation.

Purpose of the Study:

  • To investigate the effect of SOCS3 overexpression on myotube maturation in primary human skeletal muscle cells.
  • To determine SOCS3's role in regulating STAT3-mediated gene expression during myogenesis.

Main Methods:

  • Isolation and culture of primary human skeletal muscle cells.
  • Adenoviral overexpression of SOCS3.
  • Analysis of mRNA expression for STAT3 target genes (c-FOS, VEGF) and muscle maturation/hypertrophy markers.

Main Results:

  • STAT3 inhibition did not affect myotube development or survival.
  • SOCS3 overexpression enhanced mRNA expression of c-FOS and VEGF.
  • Increased expression of genes associated with muscle maturation and hypertrophy was observed.

Conclusions:

  • SOCS3 influences myoblast differentiation and myotube maturation.
  • SOCS3 may play a significant role in regulating STAT3 target genes involved in muscle development.
  • The findings suggest a complex interplay between SOCS3 and the JAK/STAT pathway in skeletal muscle.

More Related Videos

Adult and Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells14:36

Adult and Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells

Published on: September 21, 2010

Isolation of Skeletal Muscle Satellite Cells for In Vitro Myogenesis Studies07:56

Isolation of Skeletal Muscle Satellite Cells for In Vitro Myogenesis Studies

Published on: February 24, 2026

Related Concept Videos

Satellite Stem Cells and Muscular Dystrophy01:21

Satellite Stem Cells and Muscular Dystrophy

Satellite stem cells or myosatellite cells are quiescent stem cells that Alexander Mauro first identified in 1961. These cells are located between the sarcolemma, the plasma membrane of muscle fibers, and the basal lamina, the connective tissue sheath covering it. These mononucleated cells are activated in response to muscle injury, can transform into myoblasts, and may form or repair muscle fibers. Myosatellite cells can provide additional myonuclei for muscle regeneration or return to a...
Formation of Muscle Fibers from Myoblasts01:13

Formation of Muscle Fibers from Myoblasts

De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription factors...