Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Whole cross-sectional human ultrasound tomography.

Nature biomedical engineering·2026
Same author

Rotational ultrasound and photoacoustic tomography of the human body.

Nature biomedical engineering·2026
Same author

Rotational ultrasound and photoacoustic tomography of the human body.

ArXiv·2025
Same author

Whole-Body Human Ultrasound Tomography.

Research square·2024
Same author

Quantification of Cervical Elasticity During Pregnancy Based on Transvaginal Ultrasound Imaging and Stress Measurement.

IEEE transactions on bio-medical engineering·2024
Same author

Photoacoustic imaging of the dynamics of a dye-labeled IgG4 monoclonal antibody in subcutaneous tissue reveals a transient decrease in murine blood oxygenation under anesthesia.

Journal of biomedical optics·2023

Related Experiment Video

Updated: Jun 2, 2026

Three-dimensional Optical-resolution Photoacoustic Microscopy
08:31

Three-dimensional Optical-resolution Photoacoustic Microscopy

Published on: May 3, 2011

Second-generation optical-resolution photoacoustic microscopy with improved sensitivity and speed.

Song Hu1, Konstantin Maslov, Lihong V Wang

  • 1Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis, One Brookings Drive, St. Louis, Missouri 63130-4899, USA.

Optics Letters
|April 12, 2011
PubMed
Summary

We enhanced optical-resolution photoacoustic microscopy (OR-PAM) for deeper in vivo imaging. Our second-generation system offers improved sensitivity and faster scanning speeds for biological tissue analysis.

More Related Videos

Switchable Acoustic and Optical Resolution Photoacoustic Microscopy for In Vivo Small-animal Blood Vasculature Imaging
10:17

Switchable Acoustic and Optical Resolution Photoacoustic Microscopy for In Vivo Small-animal Blood Vasculature Imaging

Published on: June 26, 2017

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography
11:21

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Published on: January 15, 2013

Related Experiment Videos

Last Updated: Jun 2, 2026

Three-dimensional Optical-resolution Photoacoustic Microscopy
08:31

Three-dimensional Optical-resolution Photoacoustic Microscopy

Published on: May 3, 2011

Switchable Acoustic and Optical Resolution Photoacoustic Microscopy for In Vivo Small-animal Blood Vasculature Imaging
10:17

Switchable Acoustic and Optical Resolution Photoacoustic Microscopy for In Vivo Small-animal Blood Vasculature Imaging

Published on: June 26, 2017

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography
11:21

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Published on: January 15, 2013

Area of Science:

  • Biomedical Optics
  • Photoacoustic Imaging
  • Microscopy

Background:

  • Photoacoustic microscopy (PAM) is a powerful biomedical imaging technique.
  • Existing systems face limitations in sensitivity and imaging speed.
  • Advancements are needed for deeper in vivo tissue visualization.

Purpose of the Study:

  • To develop a second-generation (G2) optical-resolution photoacoustic microscopy (OR-PAM) system.
  • To enhance sensitivity and in vivo tissue penetration.
  • To improve scanning speed and field of view for biological imaging.

Main Methods:

  • Developed a novel acoustic detection scheme for improved sensitivity.
  • Implemented a system redesign to translate the imaging head for faster scanning.
  • Utilized 570 nm wavelength for in vivo imaging.

Main Results:

  • Achieved an 18.4 dB improvement in sensitivity compared to the first-generation (G1) system.
  • Enabled in vivo tissue penetration up to 1.2 mm at 570 nm.
  • Increased scanning speed by a factor of 5, widening the field of view.

Conclusions:

  • The G2 OR-PAM system demonstrates significantly enhanced performance for in vivo imaging.
  • The improved system allows for deeper tissue visualization and faster data acquisition.
  • Successfully imaged mouse ears and intact mouse brains, assessing hemoglobin concentration and oxygen saturation.