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Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging
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Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging

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Genomic profiling of decreased DNA damage response in human squamous carcinoma cells

Tony K S Ku1, David L Crowe

  • 1University of Illinois Cancer Center, Chicago, IL 60612, USA.

Molecular Medicine Reports
|April 12, 2011
PubMed

Insights

Chemotherapy and radiation resistance in cancer is a major challenge. This study identified new genes and response differences in sensitive versus resistant cancer cells, offering insights into overcoming treatment resistance.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genomics

Background:

  • Chemotherapy and radiation resistance in recurrent and metastatic tumors is a significant clinical problem.
  • Mechanisms of resistance include DNA damage tolerance, P-glycoprotein efflux pumps, glutathione-S-X involvement, and proto-oncogene overexpression (AP-1, c-myc, ras).

Purpose of the Study:

  • To investigate global gene expression differences between sensitive and resistant cancer cells.
  • To understand how sensitive and resistant cells respond to various types of DNA damage.

Main Methods:

  • Global gene expression profiling was employed to compare sensitive and resistant cell lines.
  • Analysis focused on differential gene responsiveness to different DNA damaging agents.

Main Results:

  • Sensitive normal epidermal keratinocytes exhibited a greater number of responsive genes compared to resistant squamous cell carcinoma cells, irrespective of DNA damage type.
  • Identified novel genes potentially involved in chemotherapy resistance.
  • Observed distinct response patterns to specific DNA damage types between sensitive and resistant cells.

Conclusions:

  • Gene expression patterns differ significantly between sensitive and resistant cancer cells.
  • Understanding these differences and cellular responses to DNA damage can reveal new therapeutic targets for overcoming resistance.

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