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Inhibiting ERp29 expression enhances radiosensitivity in human nasopharyngeal carcinoma cell lines
1Department of Otolaryngology, Xiangya Hospital, Central South University, Xiangya Road 87, Changsha 410008, Hunan Province, People's Republic of China.
Abstract:
ERp29 is an endoplasmic reticulum (ER) stress-inducible protein. It was found that ERp29 was highly expressed in several cancers and associated with resistance to oxidative and radiation stress, which may serve as a novel target for nasopharyngeal carcinoma (NPC) anticancer approach. In this study, we used immunohistochemistry to detect ERp29 expression in radioresistant and radiosensitive NPC tissues. As a result, ERp29 was up-regulated in radioresistant NPC tissues compared to radiosensitive NPC tissues. We also found that ERp29 knockdown attenuated radioresistance of NPC CNE-1 cells and ERp29 overexpression enhanced radioresistance of NPC CNE-2 cells. When exposed to radiation, ERp29 knockdown CNE-1 cells increased radiation-induced cell apoptosis and ERp29 overexpression CNE-2 cells reduced radiation-induced cell apoptosis. Further, we demonstrated that ERp29 up-regulated the expression of Hsp27. In conclusion, our study supports ERp29 could potentiate resistance to radiation in NPC cells, targeting of ERp29 is a rational strategy in treating radioresistant NPC.
Insights
Endoplasmic reticulum protein 29 (ERp29) promotes radioresistance in nasopharyngeal carcinoma (NPC). Targeting ERp29 may improve anticancer strategies for radioresistant NPC, enhancing treatment efficacy.
Area of Science:
- Oncology
- Molecular Biology
- Cellular Stress Response
Background:
- Endoplasmic reticulum protein 29 (ERp29) is induced by endoplasmic reticulum stress.
- ERp29 is overexpressed in various cancers and linked to resistance against oxidative and radiation stress.
- ERp29 presents a potential therapeutic target for nasopharyngeal carcinoma (NPC).
Purpose of the Study:
- To investigate the role of ERp29 in radioresistance of nasopharyngeal carcinoma.
- To determine ERp29 expression levels in radioresistant versus radiosensitive NPC tissues.
- To elucidate the impact of ERp29 modulation on NPC cell response to radiation therapy.
Main Methods:
- Immunohistochemistry was employed to assess ERp29 expression in NPC tissues.
- ERp29 knockdown and overexpression were performed in NPC cell lines (CNE-1 and CNE-2).
- Cell apoptosis and Hsp27 expression were analyzed following radiation exposure.
Main Results:
- ERp29 expression was significantly higher in radioresistant NPC tissues compared to radiosensitive ones.
- ERp29 knockdown decreased NPC cell radioresistance, increasing radiation-induced apoptosis.
- ERp29 overexpression enhanced NPC cell radioresistance, reducing radiation-induced apoptosis and upregulating Hsp27.
Conclusions:
- ERp29 significantly contributes to radiation resistance in nasopharyngeal carcinoma cells.
- Targeting ERp29 represents a promising therapeutic strategy for overcoming radioresistance in NPC.
- ERp29's role in potentiating radioresistance warrants further investigation for clinical applications.
