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Updated: Jun 2, 2026
Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
Published on: April 12, 2019
No effects of pulsed electromagnetic fields on expression of cell adhesion molecules (integrin, CD44) and matrix
Daguang Zhang1, Xin Pan, Shuichiro Ohno
1Department of Orthopaedic Surgery, Aichi Medical University School of Medicine, Nagakute, Aich, Japan.
Abstract:
Pulsed electromagnetic fields (PEMF) could enhance the cytocidal effects of chemotherapeutic drugs on malignant tumor cell lines, but metastasis effects of PEMF on tumor cells have not been investigated. We investigated the effects of PEMF exposure on the expression levels of some metastasis-related molecules, including integrin α subunits (α1, α2, α3, α4, α5, α6, αv), integrin β subunits (β1, β2, β3, β4), CD44, and matrix metalloproteinase-2/9 (MMP-2/9) in four human osteosarcoma cell lines (HOS, MG-63, SAOS-2, NY) and two mouse osteosarcoma cell lines (DOS, LM8) by using FACScan analysis, gelatin zymography, and Western blot analysis. Our results indicate that PEMF exposure has no effect on the expression of some molecules that are associated with tumor cell invasion and metastasis, and therefore suggest that PEMF exposure may be safely applied to chemotherapy for osteosarcoma.
Insights
Pulsed electromagnetic fields (PEMF) do not affect molecules linked to tumor cell metastasis in osteosarcoma. This suggests PEMF can be safely used alongside chemotherapy for osteosarcoma treatment.
Area of Science:
- Oncology
- Biophysics
- Biochemistry
Background:
- Pulsed electromagnetic fields (PEMF) show potential in enhancing chemotherapy's cancer cell-killing effects.
- The impact of PEMF on tumor cell metastasis, however, remains largely unexamined.
Purpose of the Study:
- To investigate the effects of PEMF exposure on metastasis-related molecules in osteosarcoma cell lines.
- To determine the safety of using PEMF in conjunction with chemotherapy for osteosarcoma.
Main Methods:
- Utilized four human (HOS, MG-63, SAOS-2, NY) and two mouse (DOS, LM8) osteosarcoma cell lines.
- Assessed expression levels of integrin subunits (α1-α6, αv, β1-β4), CD44, and matrix metalloproteinase-2/9 (MMP-2/9).
- Employed FACScan analysis, gelatin zymography, and Western blot analysis.
Main Results:
- PEMF exposure did not alter the expression of investigated molecules associated with tumor cell invasion and metastasis.
- Key metastasis markers, including integrins, CD44, and MMP-2/9, remained unaffected by PEMF treatment.
Conclusions:
- PEMF exposure does not appear to influence the expression of molecules critical for osteosarcoma cell invasion and metastasis.
- Findings support the potential for safe integration of PEMF with chemotherapy in osteosarcoma treatment regimens.
