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High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes
Published on: March 24, 2015
A convenient assay for detection of soluble interferon receptors
1Department of Molecular Genetics and Microbiology, UMDNJ-Robert Wood Johnson Medical School, Piscataway.
Journal of Biological Regulators and Homeostatic Agents
|April 1, 1990
Abstract:
The most common problem that must be overcome in purification of plasma membrane receptors from a cell line or a tissue is the loss of activity after solubilization with a detergent. This paper describes a simple dot blot assay with 32P-labeled human interferon gamma (Hu-IFN-gamma) for the detection of a functionally active Hu-IFN-gamma receptor from soluble plasma membranes prepared from human placenta.
