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Related Concept Videos

Bacterial Phylum Chlamydiae01:29

Bacterial Phylum Chlamydiae

The phylum Chlamydiae or Chlamydiota is composed of a single order, Chlamydiales. This phylum consists entirely of obligate intracellular parasites that infect eukaryotic hosts. While human pathogens within this group have been studied extensively, the phylum encompasses many species capable of interacting with various eukaryotic organisms. Members of Chlamydiae are typically small cocci, approximately 0.5 μm in diameter, and exhibit a distinctive developmental cycle. As is characteristic of...
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

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Related Experiment Video

Updated: Jun 2, 2026

Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis
10:32

Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis

Published on: June 10, 2020

Generation of targeted Chlamydia trachomatis null mutants.

Laszlo Kari1, Morgan M Goheen, Linnell B Randall

  • 1Laboratory of Intracellular Parasites, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA. karil@niaid.nih.gov

Proceedings of the National Academy of Sciences of the United States of America
|April 13, 2011
PubMed
Summary

Researchers developed a new genetic system for Chlamydia trachomatis, a major bacterial pathogen. This breakthrough enables the creation of specific mutants, advancing control strategies for trachoma and STDs.

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Forward Genetic Approaches in Chlamydia trachomatis
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Forward Genetic Approaches in Chlamydia trachomatis

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Last Updated: Jun 2, 2026

Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis
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Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis

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Markerless Gene Deletion by Floxed Cassette Allelic Exchange Mutagenesis in Chlamydia trachomatis
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Markerless Gene Deletion by Floxed Cassette Allelic Exchange Mutagenesis in Chlamydia trachomatis

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Forward Genetic Approaches in Chlamydia trachomatis
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Forward Genetic Approaches in Chlamydia trachomatis

Published on: October 23, 2013

Area of Science:

  • Microbiology
  • Bacterial Genetics
  • Pathogen Research

Background:

  • Chlamydia trachomatis is a global pathogen causing blindness and STDs.
  • Effective control is limited by the absence of a viable genetic system.
  • Developing genetic tools is crucial for understanding and combating C. trachomatis.

Purpose of the Study:

  • To establish a novel reverse-genetic system for C. trachomatis.
  • To enable the creation of isogenic mutants for functional studies.
  • To overcome limitations in chlamydial genetic manipulation.

Main Methods:

  • Ethyl methanesulfonate mutagenesis to induce low-frequency mutations.
  • Screening of mutagenized subpopulations using CEL I endonuclease.
  • Sequencing and plaque-cloning of desired mutants.
  • De novo genome sequencing to confirm isogenicity.

Main Results:

  • Successfully created an isogenic C. trachomatis trpB null mutant.
  • The trpB mutant demonstrated sensitivity to IFN-γ-induced tryptophan starvation.
  • Validated the efficacy of the reverse-genetic approach.

Conclusions:

  • The developed genetic system is a significant advancement for C. trachomatis research.
  • This methodology will accelerate the understanding of chlamydial pathogenesis.
  • The strategy holds potential for application to other challenging bacterial pathogens.