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Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography
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Performance comparison between 8- and 14-bit-depth imaging in polarization-sensitive swept-source optical coherence

Zenghai Lu1, Deepa K Kasaragod, Stephen J Matcher

  • 1Department of Materials Science and Engineering, the Kroto Research Institute, University of Sheffield, North Campus, Broad Lane, Sheffield, S3 7HQ, UK.

Biomedical Optics Express
|April 13, 2011
PubMed
Summary

True 8-bit data acquisition in swept-source optical coherence tomography (SS-OCT) shows comparable sensitivity to 14-bit systems. This suggests 8-bit systems can increase imaging speed and reduce storage for SS-OCT and polarization-sensitive SS-OCT (PS-SS-OCT).

Keywords:
(110.4280) Noise in imaging system(170.3890) Medical optics instrumentation(170.4500) Optical Coherence Tomography(260.5430) Polarization

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Area of Science:

  • Biomedical Optics
  • Medical Imaging Technology
  • Optical Coherence Tomography

Background:

  • Previous studies evaluated reduced bit-depth acquisition in swept-source optical coherence tomography (SS-OCT) using simulations and truncated higher bit-depth data.
  • These studies indicated minimal signal-to-noise ratio (SNR) drop with 8-bit acquisition compared to higher bit-depth systems.
  • However, native 8-bit analog-to-digital converters (ADCs) may have lower true bit resolution due to electronic jitter.

Purpose of the Study:

  • To directly compare true 8-bit and 14-bit depth imaging in SS-OCT and polarization-sensitive SS-OCT (PS-SS-OCT).
  • To assess the system sensitivity and image quality of native 8-bit versus 14-bit data acquisition.
  • To determine the feasibility of using 8-bit data acquisition boards for clinical SS-OCT/PS-SS-OCT systems.

Main Methods:

  • Utilized two hardware-synchronized high-speed data acquisition (DAQ) boards for simultaneous 8-bit and 14-bit data capture.
  • Acquired identical imaging data for direct comparison between the two bit depths.
  • Evaluated ex-vivo structural and birefringence images of equine tendon.

Main Results:

  • System sensitivity at 8-bit depth was comparable to 14-bit acquisition when utilizing the full analog input voltage range of the ADC.
  • Ex-vivo structural and birefringence images showed no significant differences between 8-bit and 14-bit acquired data.
  • A potential disadvantage of 8-bit acquisition is a reduced imaging dynamic range, possibly increasing artifacts from strong Fresnel reflections.

Conclusions:

  • Native 8-bit data acquisition boards are suitable for SS-OCT and PS-SS-OCT, offering comparable sensitivity to 14-bit systems.
  • Employing 8-bit DAQ can enhance imaging speeds and reduce data storage requirements in clinical applications.
  • Careful consideration of potential increases in image artifacts due to reduced dynamic range is necessary.