Related Experiment Video
Updated: Jun 2, 2026
Distinctive Capillary Action by Micro-channels in Bone-like Templates can Enhance Recruitment of Cells for Restoration of Large Bony Defect
Published on: September 11, 2015
The effect of hydroxyapatite-coated titanium fiber web on human osteoblast functional activity
Makoto Hirota1, Tohru Hayakawa, Akihiro Ametani
1Department of Oral and Maxillofacial Surgery, Yokohama City University Graduate School of Medicine, Kanazawa-ku, Yokohama, Japan. mhirota@med.yokohama-cu.ac.jp
Purpose:
The aim of this study was to evaluate human osteoblast activity on thin hydroxyapatite (HA)-coated three-dimensional scaffolds made of titanium fiber web.
Materials And Methods:
A thin HA film was coated on a titanium fiber web by the molecular precursor method. Human osteoblasts were disseminated onto the uncoated and HA-coated titanium fiber web, and osteoblast activity was observed at days 3, 7, 14, and 21 of culture.
Results:
Proliferation activities of osteoblasts were significantly higher in the uncoated titanium fiber web. Osteoblasts in the uncoated titanium fiber web showed a typical expression pattern, but those in the HA-coated titanium fiber web showed rapid osteocalcin expression and calcification at an early stage of culture. Moreover, osteocalcin expression per osteoblast was significantly higher in the HA-coated group.
Conclusion:
These results suggest that HA coating with the molecular precursor method accelerated osteoblast functional activity.
