Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Antiplatelet Drugs: Prostaglandin Synthesis, P2Y12 and Glycoprotein IIb/IIIa Inhibitors01:20

Antiplatelet Drugs: Prostaglandin Synthesis, P2Y12 and Glycoprotein IIb/IIIa Inhibitors

Antiplatelet drugs emerge as frontline defenders against the insidious threat of thromboembolic diseases, where abnormal clots obstruct vital blood vessels. These drugs stand as bulwarks, inhibiting platelet aggregation and clot formation, thereby mitigating the risk of life-threatening conditions like myocardial infarction, coronary artery disease, and thrombotic strokes.
Prostaglandin synthesis inhibitors, exemplified by the widely known aspirin, wield their power by irreversibly acetylating...
Formation of the Platelet Plug01:22

Formation of the Platelet Plug

The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss.
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
Structure and Function of Platelets01:18

Structure and Function of Platelets

The cell fragments known as platelets are disc-shaped, with an average diameter of about 3 μm and a thickness of roughly 1 μm. They play a crucial role in the body's vascular clotting system, which also involves plasma proteins, blood cells, and blood vessel tissues.
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000 platelets, with...
Transducer Mechanism: Enzyme-Linked Receptors01:27

Transducer Mechanism: Enzyme-Linked Receptors

Enzyme-linked receptors are cell-surface receptors acting as an enzyme or associating with an enzyme intracellularly. They make excellent drug targets. Drugs can bind to the extracellular ligand-binding domain or directly affect their enzymatic domain and alter their activity.
Major types that are helpful drug targets include:
Adrenergic Receptors: ɑ Subtype01:31

Adrenergic Receptors: ɑ Subtype

Adrenoceptors are classified into α and ꞵ classes based on their potencies to catecholamine agonists. α-adrenoceptors show the following order of catecholamine potency:
Adrenaline ≥ Noradrenaline >> Isoprenaline
α-adrenoceptors are further divided into α1 and α2-adrenoceptors.
α1-Adrenoceptors: These receptors are located postsynaptically on the effector organs and cause constriction of smooth muscle mediated by activation of phospholipase C—inositol-1,4,5-trisphosphate...
IP3/DAG Signaling Pathway01:11

IP3/DAG Signaling Pathway

Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the  phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and produces two-second...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Holistic management of juvenile idiopathic arthritis across all ages: British Society for Rheumatology Guideline scope.

Rheumatology advances in practice·2026
Same author

Contrast sensitivity and subjective visual disturbances across the psychosis continuum.

Cognitive neuropsychiatry·2026
Same author

A Quality Improvement Initiative to Optimize Fever and Neutropenia Management in Oncology Patients.

Pediatric quality & safety·2026
Same author

Phenome-wide association study of uterine fibroid subtypes reveals symptoms of fibroid severity and subtype-specific comorbidities.

Research square·2026
Same author

Beyond the scalpel: redefining surgical training for tomorrow: Canadian Conference for the Advancement of Surgical Education, Oct. 16-17, 2025 Winnipeg, Manitoba.

Canadian journal of surgery. Journal canadien de chirurgie·2026
Same author

The minimal important deterioration of the incremental shuttle walk test in chronic obstructive pulmonary disease: a prospective cohort study.

Annals of the American Thoracic Society·2026
Same journal

Responsiveness to adenosine diphosphate on carotid arterial and platelet functions in type 2 diabetic Otsuka Long-Evans Tokushima Fatty rats.

Purinergic signalling·2026
Same journal

The effect of P2RX7 functional SNPs on osteoblast cell phenotype, function and signalling- roles in bone homeostasis and osteogenesis.

Purinergic signalling·2026
Same journal

Selective P2X3 versus dual P2X2/3 receptor antagonists in refractory chronic cough: a systematic review and dose-response meta-analysis of randomized controlled trials.

Purinergic signalling·2026
Same journal

A tale of two ATPs: biased activation of the P2X3 receptor.

Purinergic signalling·2026
Same journal

Correction to: The analgesic effect of electroacupuncture in alleviating paclitaxel-induced peripheral neuropathic pain and its possible mechanism relates to TLR4/P2X7-NLRP3 signaling pathway.

Purinergic signalling·2026
Same journal

Adenosine A2A receptor signaling in neuroinflammation, glial modulation, and mechanisms associated with chronic pain.

Purinergic signalling·2026
See all related articles

Related Experiment Video

Updated: Jun 2, 2026

Turbidimetry on Human Washed Platelets: The Effect of the Pannexin1-inhibitor Brilliant Blue FCF on Collagen-induced Aggregation
09:13

Turbidimetry on Human Washed Platelets: The Effect of the Pannexin1-inhibitor Brilliant Blue FCF on Collagen-induced Aggregation

Published on: April 6, 2017

The P2X1 receptor and platelet function.

Martyn P Mahaut-Smith1, Sarah Jones, Richard J Evans

  • 1Department of Cell Physiology and Pharmacology, University of Leicester, Leicester, LE1 9HN, UK, mpms1@le.ac.uk.

Purinergic Signalling
|April 13, 2011
PubMed
Summary
This summary is machine-generated.

Platelet P2X1 receptors amplify thrombosis by responding to extracellular ATP. Targeting these receptors may reduce thrombotic events, particularly near atherosclerotic plaques.

More Related Videos

Real-time Live-cell Flow Cytometry to Investigate Calcium Influx, Pore Formation, and Phagocytosis by P2X7 Receptors in Adult Neural Progenitor Cells
11:47

Real-time Live-cell Flow Cytometry to Investigate Calcium Influx, Pore Formation, and Phagocytosis by P2X7 Receptors in Adult Neural Progenitor Cells

Published on: April 3, 2019

Proteomics to Identify Proteins Interacting with P2X2 Ligand-Gated Cation Channels
16:36

Proteomics to Identify Proteins Interacting with P2X2 Ligand-Gated Cation Channels

Published on: May 18, 2009

Related Experiment Videos

Last Updated: Jun 2, 2026

Turbidimetry on Human Washed Platelets: The Effect of the Pannexin1-inhibitor Brilliant Blue FCF on Collagen-induced Aggregation
09:13

Turbidimetry on Human Washed Platelets: The Effect of the Pannexin1-inhibitor Brilliant Blue FCF on Collagen-induced Aggregation

Published on: April 6, 2017

Real-time Live-cell Flow Cytometry to Investigate Calcium Influx, Pore Formation, and Phagocytosis by P2X7 Receptors in Adult Neural Progenitor Cells
11:47

Real-time Live-cell Flow Cytometry to Investigate Calcium Influx, Pore Formation, and Phagocytosis by P2X7 Receptors in Adult Neural Progenitor Cells

Published on: April 3, 2019

Proteomics to Identify Proteins Interacting with P2X2 Ligand-Gated Cation Channels
16:36

Proteomics to Identify Proteins Interacting with P2X2 Ligand-Gated Cation Channels

Published on: May 18, 2009

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Physiology

Background:

  • Extracellular nucleotides act as signaling molecules via P2 receptors.
  • Platelets express P2Y1, P2Y12, and P2X1 receptors.
  • P2X1 receptors are ATP-gated cation channels involved in platelet function.

Purpose of the Study:

  • To investigate the role of platelet P2X1 receptors in thrombosis.
  • To explore the amplification of platelet aggregation by P2X1.
  • To assess the therapeutic potential of targeting P2X1 receptors.

Main Methods:

  • In vitro studies of platelet aggregation under flow conditions.
  • In vivo studies using transgenic murine models.
  • Analysis of P2X1 receptor contribution to thrombosis and thromboembolism.

Main Results:

  • Platelet P2X1 receptors amplify Ca(2+) increases, shape change, and integrin activation.
  • P2X1 activation by released ATP enhances aggregation to collagen and thrombin.
  • P2X1 receptors amplify localized thrombosis and contribute to collagen/adrenaline-induced thromboembolism.
  • P2X1's role in aggregate formation increases with shear rate.

Conclusions:

  • Platelet P2X1 receptors play a significant role in amplifying thrombotic events.
  • P2X1 receptor activity is shear-dependent, contributing to thrombosis in high-flow areas.
  • Anti-P2X1 therapy is a potential strategy for reducing thrombotic events at atherosclerotic plaques.