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Related Concept Videos

Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
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Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
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DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

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Related Experiment Video

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Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
12:02

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Published on: June 6, 2017

An emerging model for BAP1's role in regulating cell cycle progression.

Ziad M Eletr1, Keith D Wilkinson

  • 1Department of Biochemistry, Emory University, Atlanta, GA 30322, USA.

Cell Biochemistry and Biophysics
|April 13, 2011
PubMed
Summary
This summary is machine-generated.

BRCA1-associated protein-1 (BAP1), a deubiquitinating enzyme, influences cell proliferation. BAP1 interacts with host cell factor 1 (HCF-1) to regulate gene transcription at the G1/S cell cycle transition.

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Cancer Biology

Background:

  • BRCA1-associated protein-1 (BAP1) is a nuclear deubiquitinating enzyme with tumor suppressor functions.
  • Altered BAP1 levels or activity cause cell cycle defects, particularly at the G1/S transition.
  • BAP1 interacts with the transcriptional regulator host cell factor 1 (HCF-1).

Purpose of the Study:

  • To investigate the role of BAP1 in cell cycle regulation.
  • To elucidate the functional relationship between BAP1 and HCF-1.
  • To explore how BAP1/HCF-1 complex influences transcription and cell proliferation.

Main Methods:

  • Studied BAP1's role in NCI-H226 lung carcinoma cells.
  • Investigated BAP1/HCF-1 interaction mechanisms.
  • Examined HCF-1 deubiquitination by BAP1.
  • Analyzed BAP1's effect on cell cycle progression and gene transcription.

Main Results:

  • BAP1 deubiquitinates HCF-1 in ectopic studies.
  • HCF-1 is a chromatin-associated protein involved in transcriptional regulation.
  • HCF-1 promotes cell cycle progression at G1/S by recruiting histone methyltransferases to E2F1.
  • BAP1/HCF-1 association suggests BAP1's role in co-regulating HCF-1/E2F-governed promoters.

Conclusions:

  • BAP1 likely influences cell proliferation at G1/S.
  • BAP1 may co-regulate transcription via the HCF-1/E2F pathway.
  • This interaction is a potential target for understanding lung cancer progression.