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Related Experiment Video

Updated: Jun 2, 2026

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

Stem cell genome-to-systems biology.

Na-Yu Chia¹, Huck-Hui Ng

¹Gene Regulation Laboratory, Genome Institute of Singapore, Singapore.

Wiley Interdisciplinary Reviews. Systems Biology and Medicine

|April 13, 2011

Summary

Systems biology enhances understanding of stem cell self-renewal and differentiation. By integrating experimental and computational methods, researchers map the complex regulatory networks essential for regenerative medicine applications.

Area of Science:

Stem cell biology
Systems biology
Genomics

Background:

Stem cells possess unique self-renewal and differentiation capabilities.
Understanding stem cell molecular mechanisms is key for regenerative medicine.
The genome's role in stem cell behavior requires detailed investigation.

Purpose of the Study:

To elucidate the molecular processes governing stem cell self-renewal and differentiation.
To enhance the understanding of stem cell biology using systems biology approaches.
To construct the transcriptional regulatory network of stem cells.

Main Methods:

Employing multidisciplinary approaches combining high-throughput experiments with computational and mathematical analysis.
Determining molecular constituents that control stem cell characteristics.

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Generation and Downstream Analysis of Single-Cell and Single-Nuclei Transcriptomes in Brain Organoids

Published on: March 29, 2024

Related Experiment Videos

Last Updated: Jun 2, 2026

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation

Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation

Published on: February 2, 2016

Generation and Downstream Analysis of Single-Cell and Single-Nuclei Transcriptomes in Brain Organoids

Generation and Downstream Analysis of Single-Cell and Single-Nuclei Transcriptomes in Brain Organoids

Published on: March 29, 2024

Conducting functional validations through genetic perturbation and protein location binding analysis.

Main Results:

Elucidation of protein-protein interactions.
Mapping of protein-DNA regulation.
Identification of microRNA involvement and epigenetic modifications.

Conclusions:

Systems biology provides a comprehensive understanding of stem cell features.
The transcriptional regulatory network is crucial for defining stem cell properties.
This research advances the application of stem cells in regenerative medicine.