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Updated: Jun 2, 2026
Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris
Published on: February 25, 2010
[Construstion of IL3-PE38KDEL fusion gene eukaryolic expression vector and its expression in pichia]
1Department of Hematology, the Second Affiliated Hospital, Chongqing Medical University, Chongping, China.
Aim:
to construct a eukaryolic expression plasmid of Ppic9k-IL3-Linker-PE38KDE.
Methods:
the IL3 and PE38KDEL gene were amplified by polymerase chain reaction (PCR) and cloned into the eukaryolic expression plasmid Ppic9k-Linker constructed after being sequenced.The recombinant vector confirmed by restriction endonucleases digestion, coenobium PCR and DNA sequence analysis showed that the prokaryotic expression vector Ppic9k-IL3-Linker-PE38KDEL was constructed successfully.
Conclusion:
the fusion gene IL3-PE38KDEL is successfully constructed, which laid a solid foundation for the further research.
